Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch
Identification and characterization of primordial germ cells in a vocal learning Neoaves species, the zebra finch
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DOI：
10.1096/fj.201900760rr
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发表时间：
2019-12-01
期刊：
影响因子：
4.8
通讯作者：
Han, Jae Yong
中科院分区：
文献类型：
--
作者：
Jung, Kyung Min;Kim, Young Min;Han, Jae Yong
斑胸草雀是一种很有价值的语音学习动物模型。它是鸣禽的代表，特别是声音学习鸣禽，占所有鸟类的一半，以及广泛的Neoaves，占所有鸟类的95%。虽然转基因在斑胸草雀已经完成，它是一个非常低的效率的生殖系传输和效率与更容易遗传，但声音非学习物种，鸡（Galloanseriformes）。为了提高生殖系传播的斑胸草雀，我们确定和特点的原始生殖细胞（PGCs），并比较它们与鸡。我们发现这两个物种之间存在显着差异，包括斑胸草雀PGCs数量更多，在定植到性腺之前的早期胚胎中分布更广泛，定植时间较慢，并且具有不同的发育基因表达程序。我们改进了斑胸草雀PGCs的体外分离和培养条件，并将其与基因表达载体连接，整合到宿主胚胎的性腺中。我们的研究结果表明，在PGC的斑胸草雀的重要差异，并提前创建PGC介导的生殖系转基因的声乐学习物种的第一阶段。
The zebra finch has been used as a valuable vocal learning animal model for human spoken language. It is representative of vocal learning songbirds specifically, which comprise half of all bird species, and of Neoaves broadly, which comprise 95% of all bird species. Although transgenesis in the zebra finch has been accomplished, it is with a very low efficiency of germ-line transmission and far from the efficiency with a more genetically tractable but vocal nonlearning species, the chicken (a Galloanseriformes). To improve germ-line transmission in the zebra finch, we identified and characterized its primordial germ cells (PGCs) and compared them with chicken. We found striking differences between the 2 species, including that zebra finch PGCs were more numerous, more widely distributed in early embryos before colonization into the gonads, had slower timing of colonization, and had a different developmental gene-expression program. We improved conditions for isolating and culturing zebra finch PGCs in vitro and were able to transfect them with gene-expression vectors and incorporate them into the gonads of host embryos. Our findings demonstrate important differences in the PGCs of the zebra finch and advance the first stage of creating PGC-mediated germ-line transgenics of a vocal learning species.