Insulin-like growth factor-1 (IGF-1) and IGF-1 receptor (IGF-1R) expression in human lung in RDS and BPD
Insulin-like growth factor-1 (IGF-1) and IGF-1 receptor (IGF-1R) expression in human lung in RDS and BPD
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DOI：
10.1002/ppul.10415
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发表时间：
2004-02-01
影响因子：
3.1
通讯作者：
Nielsen, HC
中科院分区：
文献类型：
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作者：
Chetty, A;Andersson, S;Nielsen, HC
我们推测，与正常发育相比，IGF-1和IGF-1R蛋白在RDS的肺上皮细胞和成纤维细胞中上调，并且在BPD中进一步上调。我们用免疫组织化学方法检测了人死产尸检及RDS和BPD患者肺组织中IGF-1和IGF-R的表达。IGF-1和IGF-R在胎儿肺、RDS和BPD肺中均有表达。在RDS中，IGF-1在肺泡上皮细胞中表达，在柱状和立方状呼吸道上皮细胞中表达明显。在BPD肺组织中，气道、肺泡上皮和间质中的免疫染色均明显增强。BPD组支气管上皮细胞和支气管周围肌成纤维细胞的免疫染色指数明显高于RDS组。IGF-1R在胎肺有少量表达，主要见于间充质。RDS间质中IGF-1R表达增强。在BPD中，以支气管周围和肺泡周围间充质增加最为明显。BPD组上皮细胞和支气管周围肌成纤维细胞的IGF-1R免疫染色指数高于RDS组。IGF-1和IGF-R在胎儿发育过程中表达较低，但在RDS中表达显著上调，并在BPD中持续上调。(C)2004年Wiley-Liss公司
We hypothesize that IGF-1 and IGF-1R proteins are upregulated in lung epithelia and fibroblasts in RDS compared to normal development, and are further upregulated in BPD. We used immunohistochemistry to evaluate IGF-1 and IGF-R expression in lungs from autopsies of human stillbirths and RDS and BPD patients. IGF-1 and IGF-R immunostaining were present in fetal, RDS, and BPD lungs. In RDS, IGF-1 was present in alveolar epithelium and prominent in columnar and cuboidal airway epithelia. In BPD lungs, immunostaining was intensely increased in both airway and alveolar epithelia and in mesenchyme. The immunostaining index in bronchial epithelial cells and peribronchial myofibroblasts was significantly higher in BPD compared to RDS. IGF-1 R expression was minimal in fetal lung and found mainly in mesenchyme. IGF-1 R was increased in mesenchyme in RDS. In BPD it was especially increased in peribronchial and perialveolar mesenchyme. Immunostaining index for IGF-1 R in epithelial cells and peribronchial myofibroblasts was increased in BPD compared to RDS. IGF-1 and IGF-R expression is low during fetal development, but is acutely upregulated in RDS, and persists with further upregulation in BPD. (C) 2004 Wiley-Liss, Inc.