Skin Brightening Efficacy of Exosomes Derived from Human Adipose Tissue-Derived Stem/Stromal Cells: A Prospective, Split-Face, Randomized Placebo-Controlled Study
Skin Brightening Efficacy of Exosomes Derived from Human Adipose Tissue-Derived Stem/Stromal Cells: A Prospective, Split-Face, Randomized Placebo-Controlled Study
复制标题
DOI：
10.3390/cosmetics7040090
复制
发表时间：
2020-12-01
期刊：
影响因子：
3.3
通讯作者：
Yi, Yong Weon
中科院分区：
文献类型：
--
作者：
Cho, Byong Seung;Lee, Jinah;Yi, Yong Weon
研究表明，干细胞及其衍生物，包括条件培养基（CM），对皮肤色素沉着有抑制作用。然而，支持来自干细胞的外泌体的皮肤亮白效果的证据是缺乏的。我们研究了人脂肪组织源性间充质干细胞外泌体（asc -exosome）的体外抗色素沉着作用和体内皮肤亮白作用。采用切向流过滤法从ASCs的CM中分离外泌体。无论α -黑色素细胞刺激激素（α - msh）是否存在，asc -外泌体均可降低B16F10黑色素瘤细胞内黑色素水平。在一项前瞻性、裂脸、双盲、随机安慰剂对照研究中，对一种含有asc外泌体的化妆品配方在色素沉着的人类志愿者中进行了皮肤亮白效果的评估。与安慰剂对照组相比，含有asc外泌体的制剂在统计学上降低了黑色素含量。然而，黑色素还原活性是有限的，并随着时间的推移而减弱。进一步改善asc外泌体的有效透皮递送将有助于获得更深远的疗效。综上所述，这些结果表明asc外泌体可以作为一种药妆剂用于皮肤美白。
Studies have shown that stem cells and their derivatives, including conditioned media (CM), have inhibitory effects on skin pigmentation. However, evidence supporting the skin brightening effect of exosomes derived from stem cells is lacking. We studied the antipigmentation effect in vitro and skin brightening efficacy in vivo of exosomes derived from human adipose tissue-derived mesenchymal stem/stromal cells (ASC-exosomes). Exosomes were isolated from the CM of ASCs using the tangential flow filtration method. ASC-exosomes reduced intracellular melanin levels in B16F10 melanoma cells regardless of the presence of the alpha-melanocyte-stimulating hormone (alpha-MSH). The skin brightening efficacy of a cosmetic formulation containing ASC-exosomes was assessed in human volunteers with hyperpigmentation in a prospective, split-face, double-blind, randomized placebo-controlled study. The ASC-exosome-containing formulation statistically decreased the melanin contents compared to the placebo control. However, the melanin-reduction activity was limited and diminished along with time. A further improvement in efficient transdermal delivery of ASC-exosomes will be helpful for more profound efficacy. In summary, these results suggest that ASC-exosomes can be used as a cosmeceutical for skin brightening.