Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome
Localization of Golgi 58K protein (formiminotransferase cyclodeaminase) to the centrosome
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DOI：
10.1007/s00418-006-0166-5
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发表时间：
2006-08-01
影响因子：
2.3
通讯作者：
Takata, Kuniaki
中科院分区：
文献类型：
--
作者：
Hagiwara, Haruo;Tajika, Yuki;Takata, Kuniaki
在脊椎动物细胞中，中心体由一对中心粒和周围的中心粒周围物质组成。使用抗高尔基体58 K蛋白抗体，识别甲酰亚胺转移酶环脱氨酶（FTCD），我们研究了其定位的中心体在各种培养细胞和人输卵管分泌细胞的免疫组织化学。除了高尔基体，FTCD定位于中心体，更丰富的母亲中心粒周围。FTCD的中心体定位在整个细胞周期中持续，并且在秋水仙胺和布雷菲德菌素A诱导的高尔基体断裂后不被破坏。中心粒微管是聚谷氨酰胺化的，并且对微管蛋白解聚药物稳定。中心体中的FTCD可能与中心粒微管的多聚谷氨酰化残基相关，并可能在为中心粒提供FTCD环脱氨酶结构域产生的谷氨酸中发挥作用。
In vertebrate cells, the centrosome consists of a pair of centrioles and surrounding pericentriolar material. Using anti-Golgi 58K protein antibodies that recognize formiminotransferase cyclodeaminase (FTCD), we investigated its localization to the centrosome in various cultured cells and human oviductal secretory cells by immunohistochemistry. In addition to the Golgi apparatus, FTCD was localized to the centrosome, more abundantly around the mother centriole. The centrosome localization of FTCD continued throughout the cell cycle and was not disrupted after Golgi fragmentation, which was induced by colcemid and brefeldin A. Centriole microtubules are polyglutamylated and stable against tubulin depolymerizing drugs. FTCD in the centrosome may be associated with polyglutamylated residues of centriole microtubules and may play a role in providing centrioles with glutamate produced by cyclodeaminase domains of FTCD.