Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)
Analysis of genetic stability at SSR loci during somatic embryogenesis in maritime pine (Pinus pinaster)
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DOI：
10.1007/s00299-008-0668-9
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发表时间：
2009-01
影响因子：
6.2
通讯作者：
L. Marum;M. Rocheta;J. Marôco;M. Oliveira;C. Miguel
中科院分区：
文献类型：
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作者：
L. Marum;M. Rocheta;J. Marôco;M. Oliveira;C. Miguel
体细胞胚胎发生（SE）是一种特别感兴趣的繁殖工具，用于加速新的高性能种植股票在多变量林业中的部署。然而，应尽早评估体外繁殖植物的遗传一致性，特别是针叶树等长寿树木。本工作的主要目的是研究这种一致性的基础上，在简单重复序列（SSR）位点的遗传稳定性在体细胞胚胎发生过程中的海洋松（PinuspinasterAit.）。胚胎发生细胞系（ECL）进行组织增殖在6，14或22个月，以及胚胎再生的几个ECL，进行了分析。在7个SSR位点的遗传变异检测ECL增殖条件下的所有时间点，并在5个的52 embranes从体细胞胚回收。这五个emelophants表现出异常的表型，主要包括斜向性和顶端优势的丧失。尽管在体细胞胚胎发生衍生的植物材料中发现了变异，但在所分析的基因座处的遗传稳定性和异常胚胎表型之间没有建立相关性，存在于64%的胚胎中。利用微卫星标记可以有效地监测水稻体细胞胚胎发生过程中的突变事件。pinaster这些分子标记应该是有用的，在实施新的育种和部署战略，改善树木使用SE。
Somatic embryogenesis (SE) is a propagation tool of particular interest for accelerating the deployment of new high-performance planting stock in multivarietal forestry. However, genetic conformity in in vitro propagated plants should be assessed as early as possible, especially in long-living trees such as conifers. The main objective of this work was to study such conformity based on genetic stability at simple sequence repeat (SSR) loci during somatic embryogenesis in maritime pine (Pinus pinasterAit.). Embryogenic cell lines (ECLs) subjected to tissue proliferation during 6, 14 or 22 months, as well as emblings regenerated from several ECLs, were analyzed. Genetic variation at seven SSR loci was detected in ECLs under proliferation conditions for all time points, and in 5 out of 52 emblings recovered from somatic embryos. Three of these five emblings showed an abnormal phenotype consisting mainly of plagiotropism and loss of apical dominance. Despite the variation found in somatic embryogenesis-derived plant material, no correlation was established between genetic stability at the analyzed loci and abnormal embling phenotype, present in 64% of the emblings. The use of microsatellites in this work was efficient for monitoring mutation events during the somatic embryogenesis inP. pinaster. These molecular markers should be useful in the implementation of new breeding and deployment strategies for improved trees using SE.