Generation of mutants with developmental defects in zebrafish by ENU mutagenesis
Generation of mutants with developmental defects in zebrafish by ENU mutagenesis
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DOI：
10.1360/982004-188
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发表时间：
2004-10-01
影响因子：
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通讯作者：
Meng, AM
中科院分区：
文献类型：
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作者：
Jin, P;Tian, T;Meng, AM
斑马鱼(Danio Rerio)作为研究脊椎动物早期发育的良好模型，正受到越来越多的关注。经ENU诱变，共建立了320个F2家系。通过观察F3胚胎的形态变化，发现突变体在胚轴、胚轴、体节、头部、心脏和血液系统中存在缺陷。到目前为止，已建立了35个突变系，其中大多数突变系的轴线和体节形成异常。这些突变系为克隆突变基因和研究脊椎动物胚胎早期发育机制提供了有用的遗传资源。
As a good model for studying early development of vertebrates, zebrafish (Danio rerio) is attracting more and more attention. Following ENU mutagenesis, 320 F2 families were established. Mutants, which showed defects in epiboly, axis, somite, head, and cardiac and blood systems, were identified by observing morphological changes in F3 embryos. So far, 35 mutant lines have been established, the majority of which showed anomalies in axis and somite formation. These mutant lines provide useful genetic resources for cloning of the mutant genes and for studying mechanisms of early development of vertebrate embryos.