Staphylococci induce the production of melanoma differentiation-associated protein-7/IL-24
Staphylococci induce the production of melanoma differentiation-associated protein-7/IL-24
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DOI：
10.1556/amicr.53.2006.4.2
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发表时间：
2006-12-01
影响因子：
1.5
通讯作者：
Megyeri, Klara
中科院分区：
文献类型：
--
作者：
Buzas, Krisztina;Megyeri, Klara
Mda-7或IL-24是IL-10细胞因子家族的新成员，通过选择性凋亡对包括黑色素瘤在内的多种人类癌症具有生长抑制作用。用植物血凝素、脂多糖、IL-4或甲型流感病毒感染诱导人PBMC表达IL-24，材料与方法：用临床标本来源的葡萄球菌菌株刺激人PBMC，用RT-nested PCR和Western blot检测IL-24在转录和翻译水平的表达。所有葡萄球菌菌株均诱导IL-24 mRNA的表达。致病性和非致病性葡萄球菌均能诱导PBMC产生IL-24，但不同菌株对IL-24产生的影响存在差异。金黄色葡萄球菌或属于皮肤正常植物群的细菌（S.表皮葡萄球菌和表皮葡萄球菌。sachticus）作为刺激Mda-7蛋白产生的因子可能是研究不同皮肤病变的关键线索。
Mda-7 or IL-24, a novel member of the IL-10 cytokine family has growth inhibitory effect on several human cancers including melanoma via selective apoptosis. Expression of IL-24 could be induced in human PBMC by treatment with phytohaemagglutinin, lypopolisaccharide, IL-4 or influenza A virus infection.Materials and Methods: Human PBMC were stimulated with staphylococcal strains originating from clinical specimens, and the appearance of IL-24 at the level of transcription and translation was followed by RT-nested PCR and Western blot assays.Results: All staphylococcal strains induced IL-24 mRNA. Staphylococci, pathogenic or not, did induce the production of IL-24 in PBMC, but there was a difference between the different staphylococcal strains in their effect on the production of the IL-24 protein.Conclusions: The pathogenic S. aureus or bacteria belonging to the normal flora of the skin (S. epidermidis and S. saprophyticus) as factors stimulating the production of the Mda-7 protein may be a crucial lead in the investigation of different skin lesions.