Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.
Single-particle reconstruction of biological macromolecules in electron microscopy--30 years.
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DOI：
10.1017/s0033583509990059
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发表时间：
2009-08
影响因子：
6.1
通讯作者：
Frank, Joachim
中科院分区：
文献类型：
--
作者：
Frank, Joachim
本文介绍了作者对单粒子重建概念发展的个人描述，单粒子重建是一种大分子组装电子显微镜技术，最近取得了令人瞩目的成就。核糖体被证明是样品制备方法、冷冻电镜技术和算法开发的理想试验场，一路上的发现是丰厚的回报。冷冻电镜和单粒子重建与分类技术相结合，越来越多地揭示不受分子接触抑制的功能分子机器的动态信息。
This essay gives the author’s personal account on the development of concepts underlying single-particle reconstruction, a technique in electron microscopy of macromolecular assemblies with a remarkable record of achievements as of late. The ribosome proved to be an ideal testing ground for the development of specimen preparation methods, cryo-EM techniques, and algorithms, with discoveries along the way as a rich reward. Increasingly, cryo-EM and single-particle reconstruction, in combination with classification techniques, is revealing dynamic information on functional molecular machines uninhibited by molecular contacts.