The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5
The nucleosome remodeling complex, Snf/Swi, is required for the maintenance of transcription in vivo and is partially redundant with the histone acetyltransferase, Gcn5
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核小体重塑复合物 Snf/Swi 是维持体内转录所必需的，并且与组蛋白乙酰转移酶 Gcn5 部分冗余
作者：
P. Sudarsanam;Yixue Cao;Lena Wu;B. Laurent;F. Winston
Snf/Swi是一种核小体重塑复合物，对于克服酿酒酵母中核小体介导的转录抑制很重要。我们已经解决了Snf/Swi控制体内Snf/Swi依赖性启动子（SUC 2基因）转录的机制。通过单细胞分析，我们的结果表明，Snf/Swi是群体中每个细胞中SUC 2表达活化水平所必需的。此外，Snf/Swi是维持SUC 2转录所必需的，这表明持续的染色质重塑对于维持活跃的转录状态是必要的。最后，Snf/Swi和Gcn 5，一种组蛋白乙酰转移酶，在SUC 2转录的控制中具有部分冗余的作用，表明两种不同机制之间的功能重叠，这两种不同机制被认为是克服核小体、核小体重塑和组蛋白乙酰化的抑制。
Snf/Swi, a nucleosome remodeling complex, is important for overcoming nucleosome‐mediated repression of transcription in Saccharomyces cerevisiae. We have addressed the mechanism by which Snf/Swi controls transcription in vivo of an Snf/Swi‐dependent promoter, that of the SUC2 gene. By single‐cell analysis, our results show that Snf/Swi is required for activated levels of SUC2 expression in every cell of a population. In addition, Snf/Swi is required for maintenance of SUC2 transcription, suggesting that continuous chromatin remodeling is necessary to maintain an active transcriptional state. Finally, Snf/Swi and Gcn5, a histone acetyltransferase, have partially redundant roles in the control of SUC2 transcription, suggesting a functional overlap between two different mechanisms believed to overcome repression by nucleosomes, nucleosome remodeling and histone acetylation.
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DOI：
10.1073/pnas.95.9.4947
发表时间：
1998-04-28
影响因子：
11.1
作者：
Côté, J;Peterson, CL;Workman, JL
通讯作者：
Workman, JL
影响因子：
3.3
作者：
M. Carlson;B. Osmond;D. Botstein
通讯作者：
M. Carlson;B. Osmond;D. Botstein
含有 HMG I/Y 的阻遏物复合物和超螺旋 DNA 拓扑对于体外长程增强子依赖性转录至关重要。
影响因子：
10.5
作者：
Bagga,R;Emerson,BM
通讯作者：
Emerson,BM
DOI：
10.1073/pnas.91.8.2905
发表时间：
1994-04-12
影响因子：
11.1
作者：
PETERSON, CL;DINGWALL, A;SCOTT, MP
通讯作者：
SCOTT, MP
影响因子：
10.5
作者：
FIERING, S;NORTHROP, JP;HERZENBERG, LA
通讯作者：
HERZENBERG, LA