CHARACTERISTICS OF MOUSE LYMPHOID-CELLS PROLIFERATING INVITRO BY RECOMBINANT HUMAN INTERLEUKIN-2 (TGP-3) - COMPARISON BETWEEN NORMAL AND NUDE-MICE
CHARACTERISTICS OF MOUSE LYMPHOID-CELLS PROLIFERATING INVITRO BY RECOMBINANT HUMAN INTERLEUKIN-2 (TGP-3) - COMPARISON BETWEEN NORMAL AND NUDE-MICE
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DOI：
10.1111/j.1348-0421.1989.tb00962.x
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发表时间：
1989-01-01
影响因子：
2.6
通讯作者：
TSUKAMOTO, K
中科院分区：
文献类型：
--
作者：
HINUMA, S;NARUO, K;TSUKAMOTO, K
用重组人白细胞介素2（rIL 2）体外培养BALB/c和BALB/cnu/nu小鼠的各种淋巴细胞，分析rIL 2应答细胞的特性。从BALB/c小鼠中获得的脾细胞、淋巴结细胞和胸腺细胞除了骨髓细胞外，对rIL 2有显著的增殖反应。另一方面，在从BALB/c nu/nu小鼠获得的淋巴样细胞中，只有淋巴结细胞通过rIL 2显示出显著的增殖。流式细胞术分析显示，BALB/c小鼠中主要有两种类型的淋巴细胞响应rIL 2而增殖，即，Thy 1+、Lyt 1-、Lyt 2-和Thy 1+、Lyt 1-、Lyt 2+细胞。而BALB/c nu/nu小鼠则以Thy 1+、Lyt 1-、Lyt 2-细胞为主。用各种抗体加补体处理BALB/c小鼠后，发现大部分IL 2反应性细胞为Thy 1+、Lyt 1+和Lyt 2+，也有一小部分为Thy 1-、Lyt 1-和Lyt 2-。另一方面，BALB/c nu/nu小鼠IL 2反应细胞的主要类型为Thy 1-、Lyt 1-和Lyt 2-，也有Thy 1+。rIL 2培养后，两株淋巴样细胞对肿瘤细胞的非特异性杀伤活性均有不同程度的增强。我们的研究结果表明小鼠对rIL 2的应答细胞具有以下特征。首先，应答细胞在正常小鼠的脾脏、淋巴结和胸腺中大量存在，尽管它们的细胞谱系是异质的;一种是T细胞谱系，另一种是自然杀伤（NK）细胞谱系。第二，裸鼠体内存在T细胞系的应答细胞，而不存在NK细胞系的应答细胞。此外，裸鼠中的应答细胞主要积聚在淋巴结中，而不是其它淋巴器官中。
Various lymphoid cells obtained from BALB/c and BALB/c nu/nu mice were cultured in vitro with recombinant human interleukin 2 (rIL 2), and the characteristics of responder cells to rIL 2 were analyzed. Spleen cells, lymph node cells, and thymocytes except for bone marrow cells obtained from BALB/c mice remarkably proliferated in response to rIL 2. On the other hand, among lymphoid cells obtained from BALB/c nu/nu mice, only lymph node cells showed significant proliferation by rIL 2. Flow cytometric analyses revealed that mainly two types of lymphoid cells were proliferating in response to rIL 2 in BALB/c mice, i.e., Thy 1+, Lyt 1-, Lyt 2- and Thy 1+, Lyt 1-, Lyt 2+ cells. On the other hand, most of the proliferating cells were Thy 1+, Lyt 1-, Lyt 2- cells in BALB/c nu/nu mice. Treatment with various antibodies plus complement revealed that the majority of IL 2-responsive cells in BALB/c mice were Thy 1+, Lyt 1+, and Lyt 2+, although a minor part of them were Thy 1-, Lyt 1-, and Lyt 2-. On the other hand, a predominant type of the IL 2-responsive cells in BALB/c nu/nu mice were Thy 1-, Lyt 1-, and Lyt 2-, though some were Thy 1+. Nonspecific killer activity against tumor cells increased to variable extents in all of the lymphoid cells of both strains after culture with rIL 2. Our results indicate that mouse responder cells to rIL 2 have the following characteristics. First, the responder cells exist abundantly among spleen, lymph nodes, and thymus in normal mice, though their cell lineages are heterogeneous; one is of T cell lineage and the other of natural kiiller (NK) cell lineage. Second, nude mice are devective in the responder cells of T cell lineage but not of NK cell lineage. Moreover, the responder cells in nude mice predominantly accumulate in the lymph nodes but not other lymphoid organs.