Bursicon functions within the Drosophila CNS to modulate wing expansion behavior, hormone secretion, and cell death.
Bursicon functions within the Drosophila CNS to modulate wing expansion behavior, hormone secretion, and cell death.
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DOI：
10.1523/jneurosci.2842-08.2008
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发表时间：
2008-12-31
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影响因子：
--
通讯作者：
White BH
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文献类型：
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作者：
Peabody NC;Diao F;Luan H;Wang H;Dewey EM;Honegger HW;White BH
激素通常负责同步躯体生理变化与行为变化。昆虫的蜕皮（即外骨骼的脱落）已成为阐明这种同步化的分子和细胞机制的有用模型，并为身体和行为的激素协调提供了许多见解。其中一个机制仍然是谜的例子是神经激素bursicon，它在最后一次蜕皮后，协调最初折叠翅膀的塑化和晒黑，以及驱动翅膀扩张的行为。这种激素的躯体效应是由腹神经节（BAG）中的神经元释放到血液中的囊子控制的，这些神经元在翅膀扩张后死亡。然而，bursicon如何诱导翅膀扩张所需的行为程序仍然未知。在这里，我们表明，有针对性的抑制兴奋性，一对bursicon免疫反应神经元不同的BAG和位于食管下神经节在果蝇（BSEG）参与控制翅膀扩张行为。与BAG不同，BSEG广泛分布于神经系统中，包括腹部神经节内，这表明除了控制行为外，它们还可以调节BAG。事实上，我们表明，动物缺乏囊素受体功能有缺陷的体液释放囊素和羽化后的细胞凋亡的袋。我们的研究结果揭示了新的神经调节功能的bursicon和支持的假设，BSEG是必不可少的精心策划的行为和躯体过程的翅膀扩张。
Hormones are often responsible for synchronizing somatic physiological changes with changes in behavior. Ecdysis (i.e. the shedding of the exoskeleton) in insects has served as a useful model for elucidating the molecular and cellular mechanisms of this synchronization, and has provided numerous insights into the hormonal coordination of body and behavior. An example in which the mechanisms have remained enigmatic is the neurohormone bursicon, which after the final molt, coordinates the plasticization and tanning of the initially folded wings with behaviors that drive wing expansion. The somatic effects of the hormone are governed by bursicon that is released into the blood from neurons in the abdominal ganglion (the BAG), which die after wing expansion. How bursicon induces the behavioral programs required for wing expansion, however, has remained unknown. Here we show by targeted suppression of excitability that a pair of bursicon-immunoreactive neurons distinct from the BAG and located within the subesophageal ganglion in Drosophila (the BSEG) is involved in controlling wing expansion behaviors. Unlike the BAG, the BSEG arborize widely in the nervous system, including within the abdominal neuromeres, suggesting that, in addition to governing behavior, they also may modulate the BAG. Indeed, we show that animals lacking bursicon receptor function have deficits both in the humoral release of bursicon and in post-eclosion apoptosis of the BAG. Our results reveal novel neuromodulatory functions for bursicon and support the hypothesis that the BSEG are essential for orchestrating both the behavioral and somatic processes underlying wing expansion.