Solid-state NMR investigation of sodium nucleotide complexes.
Solid-state NMR investigation of sodium nucleotide complexes.
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钠核苷酸复合物的固态核磁共振研究。
DOI：
10.1002/mrc.1750
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Frydman,Lucio
中科院分区：
文献类型：
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作者：
Grant,ChristopherV;McElheny,Dan;Frydman,Veronica;Frydman,Lucio
固态NMR已被用于分析钠核苷酸复合物粉末晶体样品中钠位点的化学环境。研究的三种复合物先前已通过晶体学进行了结构表征（脱氧胞苷-5 ′-单磷酸二钠七水合物、脱氧尿苷-5 ′-单磷酸二钠五水合物和腺苷-5 ′-三磷酸二钠三水合物）。对于这些盐，23 Na多量子魔角自旋NMR测量的核四极耦合参数可以很容易地与钠离子配位环境相关联。此外，通过固态NMR鉴定了两种先前未在结构上表征的复合物，尿苷-3 ′-单磷酸二钠和尿苷-3 ′-单磷酸二钠/尿苷-2 ′-单磷酸二钠混合物。一个光谱分配的四个网站的一个额外的盐，腺苷-5 ′-三磷酸二钠三水合物，还介绍和讨论的背景下创建一个通用的方法，在多个网站的光谱分配的钠核苷酸复合物。版权所有© 2006约翰威利父子有限公司。
Solid‐state NMR has been used to analyze the chemical environments of sodium sites in powdered crystalline samples of sodium nucleotide complexes. Three of the studied complexes have been previously characterized structurally by crystallography (disodium deoxycytidine‐5′‐monophosphate heptahydrate, disodium deoxyuridine‐5′‐monophosphate pentahydrate and disodium adensoine‐5′‐triphosphate trihydrate). For these salts, the nuclear quadrupole coupling parameters measured by23Na multiple‐quantum magic‐angle‐spinning NMR could be readily correlated with sodium ion coordination environments. Furthermore, two complexes that had not been previously characterized structurally, disodium uridine‐3′‐monophosphate and a disodium uridine‐3′‐monophosphate/disodium uridine‐2′‐monophosphate mix, were identified by solid‐state NMR. A spectroscopic assignment of the four sites of an additional salt, disodium adensoine‐5′‐triphosphate trihydrate, is also presented and discussed within the context of creating a general approach for the spectroscopic assignment of multiple sites in sodium nucleotide complexes. Copyright © 2006 John Wiley & Sons, Ltd.