Fluorescence polarization assay for diagnosis of human brucellosis
Fluorescence polarization assay for diagnosis of human brucellosis
复制标题
DOI：
10.1099/jmm.0.05217-0
复制
发表时间：
2003-10-01
影响因子：
3
通讯作者：
Nielsen, K
中科院分区：
文献类型：
--
作者：
Lucero, NE;Escobar, GI;Nielsen, K
荧光偏振免疫分析（FPA）利用分子旋转特性直接测量抗体与抗原的结合。与竞争性酶免疫测定法（CELISA）和常规血清学试验相比，共587份人血清用于布鲁氏菌病的诊断，评估了该方法的潜在用途。基于340份来自无布鲁氏菌病证据的无症状献血者的血清，FPA的特异性为97.9%，使用72 mP的截止值。布鲁氏菌感染患者血清（羊种布鲁氏菌11例，牛种布鲁氏菌32例，猪种布鲁氏菌32例，布鲁氏菌1例）敏感性估计为96.1%。在对84份疑似布鲁氏菌病患者血清的检测中，FPA检出了80例病例。在87份疑似感染患者血清中，15份同时用CELISA和FPA检测，3份仅用CELISA，4份仅用FPA。两组中的差异涉及血清滴度较低且呈下降趋势。FPA使用40穆尔血清样品，需要大约5分钟完成，并且已经证明对于检测B抗体是准确的。abortus，牛种B. melitensis和B. suis和用于识别患有复发的患者。由于该方法简单，因此可以很容易地在临床实验室和血库中使用。
Fluorescence polarization immunoassay (FPA) uses molecular rotational properties to measure antibody binding to antigen directly. The potential use of this method was assessed in comparison to a competitive enzyme immunoassay (CELISA) and conventional serological tests for the diagnosis of brucellosis on a total of 587 human sera. Based on 340 sera from asymptomatic blood donors with no evidence of brucellosis, the specificity of the FPA was 97.9 % using a cut-off value of 72 mP. Sera from Brucella-infected patients (11 Brucella melitensis, 32 Brucella abortus, 32 Brucella suis and one Brucella sp.) yielded a sensitivity estimate of 96.1 %. In tests on 84 sera from suspected brucellosis patients, the FPA detected 80 cases. Of 87 sera from patients with probable infection, 15 were detected by both CELISA and FPA, three by CELISA only and four by FPA only. The discrepancies in both groups involved sera with low, declining titres. The FPA uses a sample of 40 mul serum, takes about 5 min to complete and has been demonstrated to be accurate for the detection of antibodies to B. abortus, B. melitensis and B. suis and for identifying patients suffering relapses. Because of the ease of the procedure, it could be readily adopted for use in clinical laboratories and blood banks.