Tumorcidal effect of recombinant Newcastle Disease Virus.
Tumorcidal effect of recombinant Newcastle Disease Virus.
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DOI：
10.2174/1874318800802010011
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发表时间：
2008-01-01
期刊：
影响因子：
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通讯作者：
Nakaya, T.
中科院分区：
文献类型：
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作者：
Hagiwara, K.;Kadosawa, T.;Nakaya, T.
我们已经基于活疫苗株产生了表达GFP的重组纽卡斯尔病病毒（rNDV-GFP），所述活疫苗株含有导致病毒体内生长受限的致病性较低的F蛋白切割位点。本实验评价了rNDV对B16黑色素瘤细胞的溶瘤作用。rNDV-GFP能有效感染B16细胞并诱导细胞死亡。凋亡相关基因Bax和Caspase 3在感染细胞中升高。为了评价rNDV对犬肿瘤细胞的杀伤作用，用rNDV感染犬高转移性骨肉瘤（HMPOS）和移行细胞癌（TCC）两种肿瘤。rNDV在感染后48小时内诱导犬肿瘤细胞的细胞死亡。这些结果表明，rNDV-GFP在体外对B16小鼠黑色素瘤和犬肿瘤具有杀瘤活性。
We have generated GFP expressing recombinant Newcastle Disease Virus (rNDV-GFP) based on a live vaccine strain which contains a less pathogenic cleavage site of F protein leading to restricted viral growth in vivo. We evaluated the rNDV have an effect of oncolytic activity to B16 melanoma cell. The rNDV-GFP infected efficiency to B16 cells and induced cell death. Apoptosis-related genes, Bax and Caspase 3, were elevated in the infected cells. In order to evaluate the tumorcidal effect of rNDV on canine tumor cells, two canine tumors such as highly metastasizing canine osteosarcoma (HMPOS) and transitional cell carcinoma (TCC) were infected with rNDV. The rNDV induced cell death to canine tumor cells within 48 hours post infection. These results suggest that rNDV-GFP have a tumorcidal activity to B16 mouse melanoma and canine tumors in vitro.