In vitro mineralization of a glass-ceramic of the MgO-3CaO • P2O-SiO2 system:: Wettability studies
In vitro mineralization of a glass-ceramic of the MgO-3CaO • P2O-SiO2 system:: Wettability studies
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DOI：
10.1002/jbm.10194
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发表时间：
2002-07-01
期刊：
影响因子：
--
通讯作者：
Fernandes, MHV
中科院分区：
文献类型：
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作者：
Serro, AP;Fernandes, AC;Fernandes, MHV
通过润湿性研究，研究了在生物模型流体中浸泡1周后，CaO-3CaO·P2O5-SiO_2系生物活性微晶玻璃表面的钙磷沉积情况。测试了两种化学成分与人体血浆相似的模型液体：汉克斯平衡盐溶液(HBSS)和钙含量较高的模拟体液(SBF)。评估了白蛋白存在的影响，无论是溶液中的白蛋白还是先前吸附到表面的白蛋白。比较了使用和不使用Tris缓冲液制备的两种SBF溶液的行为。测定了微晶玻璃样品浸泡前后的表面自由能，并以生物模型流体为扫描液体进行了动态接触角分析。扫描电子显微镜观察和X射线光电子能谱(XPS)分析是对润湿性研究的补充。实验结果表明，在HBSS中，钙磷膜的析出主要发生在每日更新浸泡液或有吸附白蛋白存在的情况下。在SBF中，Tris缓冲液的加入似乎抑制了磷酸钙膜的形成。(C)2002年威利期刊公司。
The calcium phosphate deposition on the surface of a bioactive glass-ceramic of the MgO-3CaO.P2O5-SiO2 system during a I-week immersion in biological model fluids, was investigated through wettability studies. Two model fluids with chemical composition similar to the human blood plasma were tested: Hanks' balanced salt solution (HBSS) and a simulated body fluid (SBF) with a higher calcium content. The effect of the presence of albumin, both in solution or previously adsorbed to the surface, was assessed. The behavior observed using two SBF solutions prepared with and without TRIS buffer was compared. The surface free energy of the glass-ceramic samples was determined, before and after immersion, and dynamic contact angle analysis was performed using the biological model fluids as scanning liquids. Scanning electron microscope observations and x-ray photoelectron spectroscopy (XPS) analysis were performed to complement the wettability studies. The experimental results led to the conclusion that the precipitation of a calcium phosphate film in HBSS occurred mainly when the immersion solution was renewed daily or in the presence of adsorbed albumin. In SBF, the addition of TRIS buffer seemed to inhibit the formation of the calcium phosphate film. (C) 2002 Wiley Periodicals, Inc.