Interaction of bulky chemical carcinogens with DNA in chromatin.
Interaction of bulky chemical carcinogens with DNA in chromatin.
复制标题
大体积化学致癌物与染色质 DNA 的相互作用。
DOI：
10.1093/carcin/16.9.2009
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发表时间：
1995
期刊：
影响因子：
4.7
通讯作者：
MacLeod,MC
中科院分区：
文献类型：
--
作者：
MacLeod,MC
DNA损伤是化学致癌物处理细胞和生物体的重要效应。虽然从致癌物与纯化DNA反应的体外研究中已经了解到很多，但在体内，DNA与各种组蛋白和非组蛋白蛋白在称为染色质的复杂动态结构中相关联。本文综述了大体积化学致癌物与染色质的共价相互作用。在复制、转录和核基质结合的DNA区域，以及核小体核心和接头DNA区域中的DNA之间，发现了与大量基因组DNA在加合物密度上的差异。这些差异的范围从连接子与核心的2至3倍，到接近复制叉的108倍。还有很多工作要做，以确定非组蛋白和染色质高阶结构的致癌物结合的影响。
DNA damage is an important effect of treatment of cells and organisms with chemical carcinogens. Although much has been learned fromin vitrostudies of the reaction of carcinogens with purified DNA,in vivothe DNA is associated with a variety of histone and non-histone proteins in a complex and dynamic structure known as chromatin. The covalent interactions of bulky chemical carcinogens with chromatin are reviewed. Differences from bulk genomic DNA in adduct density are found in replicating, transcribing and nuclear matrix-bound DNA regions, and between DNA in nucleosome cores and linker DNA regions. These differences range from 2- to 3-fold for linker versus core, to ∼8-fold close to a replication fork. Much remains to be done to determine the influences of non-histone proteins and higher order chromatin structure on carcinogen binding.