Detection of polyol accumulation in a new ovarian carcinoma cell line, CABA I: a 1H NMR study
Detection of polyol accumulation in a new ovarian carcinoma cell line, CABA I: a 1H NMR study
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DOI：
10.1038/sj/bjc/6600189
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发表时间：
2002-04-08
影响因子：
8.8
通讯作者：
Podo, F
中科院分区：
文献类型：
--
作者：
Ferretti, A;D'Ascenzo, S;Podo, F
卵巢癌是妇科恶性肿瘤的主要形式，其治疗主要包括手术和化疗。除了预后困难外，由于缺乏疗效和耐药性的发展，卵巢癌的治疗几乎没有改善。不同的生物化学和功能参数的需求已经增长，以获得更大的生物学和临床意义的过程的看法。在本文中，我们报告了新的代谢功能检测在一系列不同的人卵巢癌细胞系，完整的细胞和提取物的H-1 NMR光谱。最重要的是，新的卵巢腺癌细胞系CABA 1在3.5和4.0 p. p.m.之间的光谱区域显示出强信号，首次分配给多元醇山梨醇（39 +/- 11 nmol/10（6）个细胞）。用[1-C-13]-D-葡萄糖孵育的这些细胞的C-13 NMR分析表明形成了标记的山梨醇。其他卵巢癌细胞系（OVCAR-3、IGF 1、SK-OV-3和OVCA 432）在相同的光谱区域显示来自其他代谢物的强烈共振：谷胱甘肽（高达30 nMol/10（6）个细胞）和肌醇（高达50 nmol/10（6）个细胞）。这些化合物在人类卵巢癌细胞中具有生化和生物学功能，特别是与它们在肿瘤进展期间细胞解毒机制中可能发挥的作用有关。(C)2002年英国癌症研究。
Ovarian carcinomas represent a major form of gynaecological malignancies, whose treatment consists mainly of surgery and chemotherapy. Besides the difficulty of prognosis, therapy of ovarian carcinomas has reached scarce improvement, as a consequence of lack of efficacy and development of drug-resistance. The need of different biochemical and functional parameters has grown, in order to obtain a larger view on processes of biological and clinical significance. In this paper we report novel metabolic features detected in a series of different human ovary carcinoma lines, by H-1 NMR spectroscopy of intact cells and their extracts. Most importantly, a new ovarian adenocarcinoma line CABA 1, showed strong signals in the spectral region between 3.5 and 4.0 p.p.m., assigned for the first time to the polyol sorbitol (39 +/- 11 nmol/10(6) cells). C-13 NMR analyses of these cells incubated with [1-C-13]-D-glucose demonstrated labelled-sorbitol formation. The other ovarian carcinoma cell lines (OVCAR-3, IGROV 1, SK-OV-3 and OVCA432), showed, in the same spectral region, intense resonances from other metabolites: glutathione (up to 30 nMol/10(6) cells) and myo-inositol (up to 50 nmol/10(6) cells). Biochemical and biological functions are suggested for these compounds in human ovarian carcinoma cells, especially in relation to their possible role in cell detoxification mechanisms during tumour progression. (C) 2002 Cancer Research UK.