PORCINE AORTIC ENDOTHELIAL-CELLS ACTIVATE HUMAN T-CELLS - DIRECT PRESENTATION OF MHC ANTIGENS AND COSTIMULATION BY LIGANDS FOR HUMAN CD2 AND CD28
PORCINE AORTIC ENDOTHELIAL-CELLS ACTIVATE HUMAN T-CELLS - DIRECT PRESENTATION OF MHC ANTIGENS AND COSTIMULATION BY LIGANDS FOR HUMAN CD2 AND CD28
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DOI：
10.1016/1074-7613(94)90009-4
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发表时间：
1994-04-01
期刊：
影响因子：
32.4
通讯作者：
BOTHWELL, ALM
中科院分区：
文献类型：
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作者：
MURRAY, AG;KHODADOUST, MM;BOTHWELL, ALM
我们研究了人类异种对培养的猪主动脉内皮细胞（PAECs）的反应。人CD 8（+）T细胞增殖为静息的MHC I类阳性PAEC。在猪干扰素-γ诱导MHC II类分子后，CD 4（+）T细胞增殖。这些反应大于对人脐静脉内皮细胞（HUVEC）的相应同种异体反应。有限稀释分析显示，异种反应性的频率比同种异体反应性抗内皮淋巴细胞高10倍。物种特异性单克隆抗体表明PAEC直接将猪MHC抗原呈递给人T细胞，并且人CD 4和CD 8分子参与这种相互作用。此外，PAEC结合CTLA-4-Ig并通过CD 2和CD 28途径共刺激人T细胞。相比之下，HUVEC不结合CTLA-4-Ig，并且仅使用⑶ 2途径。
We examined the human xenoresponse to cultured porcine aortic endothelial cells (PAECs). Human CD8(+) T cells proliferate to resting MHC class I-positive PAECs. CD4(+) T cells proliferate after MHC class II molecules are induced with swine interferon-gamma. These responses are greater than corresponding allogeneic responses to human umbilical vein endothelial cells (HUVECs). Limiting dilution analysis shows a 10-fold higher frequency of xenoreactive than alloreactive anti-endothelial lymphocytes. Species-specific monoclonal antibodies suggest that PAECs directly present swine MHC antigens to human T cells and that human CD4 and CD8 molecules participate in this interaction. Furthermore, PAECs bind CTLA-4-lg and costimulate human T cells by both the CD2 and CD28 pathways. In contrast, HUVECs do not bind CTLA-4-lg and only use the CD2 pathway.