Differential Bvg phase-dependent regulation and combinatorial role in pathogenesis of two Bordetella paralogs, BipA and BcfA.
Differential Bvg phase-dependent regulation and combinatorial role in pathogenesis of two Bordetella paralogs, BipA and BcfA.
复制标题
两种博德特氏菌旁系同源物 BipA 和 BcfA 发病机制中的差异 Bvg 相位依赖性调节和组合作用。
DOI：
10.1128/jb.00009-07
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发表时间：
2007
影响因子：
3.2
通讯作者：
Deora,Rajendar
中科院分区：
文献类型：
--
作者：
Sukumar,Neelima;Mishra,Meenu;Sloan,GinaParise;Ogi,Tomoo;Deora,Rajendar
To successfully colonize their mammalian hosts, many bacteria produce multiple virulence factors that play essential roles in disease processes and pathogenesis. Some of these molecules are adhesins that allow efficient attachment to host cells, a prerequisite for successful host colonization.Bordetellaspp. express a number of proteins which either play a direct role in attachment to the respiratory epithelia or exhibit similarity to known bacterial adhesins. One such recently identified protein is BipA. Despite the similarity of BipA to intimins and invasins, deletion of this protein fromB. bronchisepticadid not result in any significant defect in respiratory tract colonization. In this study, we identified an open reading frame inB. bronchiseptica, designatedbcfA(encoding BcfA [bordetellacolonizationfactorA]), that is similar tobipA. In contrast to the maximal expression ofbipAin the Bvg intermediate (Bvgi) phase,bcfAis expressed at high levels in both the Bvg+and Bvgiphases. We show here that BvgA and phosphorylated BvgA bind differentially to thebcfApromoter region. Utilizing immunoblot assays, we found that BcfA is localized to the outer membrane and that it is expressed during animal infection. While deletion of eitherbipAorbcfAdid not significantly affect respiratory tract colonization, concomitant deletion of both genes resulted in a defect in colonization of the rat trachea. Our results indicate that the two paralogous proteins have a combinatorial role in mediating efficient respiratory tract colonization.
