Endothelium and "silver lines". An electron microscopic study.
Endothelium and "silver lines". An electron microscopic study.
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内皮和“银线”。
DOI：
10.1007/bf00429607
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Joris,I
中科院分区：
文献类型：
--
作者：
Zand,T;Underwood,JM;Nunnari,JJ;Majno,G;Joris,I
用透射电子显微镜观察了戊二醛加硝酸银灌流后大鼠主动脉内皮细胞“银线”的意义。标准的透射电子显微镜技术不能令人满意(银粒粗大、定位不精确、伪影)。用照相固定剂照射银处理的主动脉后，沉积的图像明显改善，留下了直径约为100？的细小、稳定、均匀的“残留颗粒”。这些颗粒大多分布在细胞间连接处；它们也往往聚集在每个连接处下面的基底膜中。这张图像表明，Ag+离子穿过结合处，并与其内容物以及其以外的基底膜发生反应。提出了一个方案来解释Ag+离子与结内的阴离子和带负电荷的自由基的反应。结论：“银线”不仅代表一种组织化学效应，而且是一种跨内皮细胞电解质途径的可视化。
The significance of endothelial “silver lines” was studied by TEM in rat aortas after perfusion with glutaraldehyde followed by silver nitrate. Standard TEM technique proved unsatisfactory (coarse silver granules, imprecise localization, artefacts). Exposure of the silver-treated aortas to photographic fixer markedly improved the image of the deposits leaving fine, stable, uniform “residual granules” about 100 Å in diameter. Most of these granules were localized along the intercellular junctions; they also tended to pool in the basement membrane beneath each junction. This image suggests that the Ag+ions pass through the junction, and react with its contents as well as with the basement membrane beyond it. A scheme is proposed to explain the reaction of Ag+ions with anions and negatively charged radicals within the junction. It is concluded that the “silver lines” represent not only a histochemical effect, but also the visualization of a transendothelial electrolyte pathway.