Induction of cross-links in corneal tissue
Induction of cross-links in corneal tissue
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DOI：
10.1006/exer.1997.0410
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发表时间：
1998-01-01
影响因子：
3.4
通讯作者：
Seiler, T
中科院分区：
文献类型：
--
作者：
Spoerl, E;Huhle, M;Seiler, T
本研究的目的是探讨在角膜组织中诱导交联链的可能性，以增加角膜组织的硬度，为将来保守治疗角膜扩张症奠定基础。胶原性生物材料可以通过化学和物理手段来稳定。摘除去核猪眼上皮。用紫外光(波长=254 nm)、0.5%核黄素、0.5%核黄素和W光(365 Nm)蓝光(436 Nm)和太阳光，以及化学试剂戊二醛(1%和0.1%，10min)和Karnovsky‘s液(0.1%，10min)治疗8个试验组，每组10只眼。从每个角膜上切下宽5 mm、长9 mm的条带，测量这些条带的应力-应变行为，以评估交联过程。为了进行比较，10个未经治疗的角膜也用同样的方法进行了测量。与未经治疗的角膜相比，用核黄素、紫外线照射以及弱戊二醛或卡诺夫斯基溶液治疗的角膜硬度增加。卡诺夫斯基溶液戊二醛、核黄素和紫外线照射可以改变角膜的生物力学行为，这为圆锥角膜的保守治疗提供了可能性。为了优化这一效应，有必要就剂量反应和体内应用进行进一步的研究。(C)1998年学术出版社有限公司。
The aim of this study was to investigate the possibility of induction of cross-links in corneal tissue in order to increase the stiffness as a basis for a future conservative treatment of keratectasia. Collagenous biomaterials can be stabilized by chemical and physical agents. The epithelium of enucleated porcine eyes was removed. Eight test groups, 10 eyes each, were treated with UV-light (lambda = 254 nm), 0.5% riboflavin, 0.5% riboflavin and W-light (365 nm) blue light (436 nm) and sunlight, and the chemical agents-glutaraldehyde (1% and 0.1%, 10 min) and Karnovsky's solution (0.1%, 10 min). Strips of 5 mm in width and 9 mm in length were cut from each cornea and the stress-strain behaviour of the strips was measured to assess the cross-linking process. For comparison, ten untreated corneas were measured by the same method. Compared to untreated corneas treatment with riboflavin and UV-irradiation as well as weak glutaraldehyde or Karnovsky's solutions resulted in an increased stiffness of the cornea. The biomechanical behaviour of the cornea can be altered by glutaraldehyde, Karnovsky's solution, and with riboflavin and UV-irradiation which offers the potential of a conservative treatment of keratoconus. To optimize this effect further investigation is necessary regarding the dose-response and in-vivo application. (C) 1998 Academic Press Limited.