Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal
Production of a bioflocculant by Aspergillus parasiticus and its application in dye removal
复制标题
DOI：
10.1016/j.colsurfb.2005.06.011
复制
发表时间：
2005-09-01
影响因子：
5.8
通讯作者：
Ting, YP
中科院分区：
文献类型：
--
作者：
Deng, SB;Yu, G;Ting, YP
寄生曲霉产生的生物絮凝剂对高岭土悬浮液和水溶性染料具有很高的絮凝活性。结果表明，该微生物絮凝剂的最佳碳源和氮源为玉米淀粉和蛋白胨，最佳产絮凝剂条件为温度28 ℃，初始pH 5-6，摇床转速150 rpm。高岭土悬浮液的最高絮凝效率为98。1%。该絮凝剂主要由糖（76.3%）和蛋白质（21.6%）组成，平均分子量为3.2 × 10 ~（5）Da，X射线光电子能谱（XPS）和傅立叶变换红外光谱（FTIR）分析表明，絮凝剂分子中存在氨基、酰胺基和羟基。该絮凝剂对水溶性阴离子染料的脱色效果较好，对活性蓝4和酸性黄25的脱色率分别为92.4%和92.9%。脱色效率取决于絮凝剂的投加量和溶液的pH值。XPS结果表明，在pH值为5时，生物絮凝剂中的胺基被质子化，从而使带正电的生物絮凝剂被吸引到带负电的染料分子上。从静电相互作用的角度出发，认为生物絮凝剂分子中的氨基和酰胺基在絮凝过程中起着重要作用。(c)2005 Elsevier B. V.保留所有权利。
Aspergillus parasiticus was found to produce a bioflocculant with high flocculating activity for Kaolin suspension and water-soluble dyes. Results showed that the carbon and nitrogen sources favorable for the production of the bioflocculant were corn starch and peptone, and an optimal condition of 28 degrees C, initial pH 5-6 and shaking speed of 150 rpm. The highest flocculating efficiency achieved for Kaolin suspension was 98. 1 %, after 72 h cultivation. The bioflocculant was mainly composed of sugar (76.3%) and protein (21.6%), and an average molecular weight of 3.2 x 10(5) Da. X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared (FTIR) spectra showed that amino, amide and hydroxyl groups were present in the bioflocculant molecules. The bioflocculant was effective in flocculating some soluble anionic dyes in aqueous solution, in particular Reactive Blue 4 and Acid Yellow 25 with a decolorization efficiency of 92.4 and 92.9%, respectively. The decolorization efficiency was dependent on the flocculant dosage and solution pH. XPS result shows that the amine groups in the bioflocculant were protonated at pH 5, and thus the positive bioflocculant was attracted to the negatively charged dye molecules. The amino and amide groups in the bioflocculant molecule are believed to play an important role in flocculation from the viewpoint of electrostatic interaction. (c) 2005 Elsevier B.V. All rights reserved.