2ND GENERATION MONOCLONAL-ANTIBODIES TO THE HUMAN INTEGRIN ALPHA-6-BETA-4
2ND GENERATION MONOCLONAL-ANTIBODIES TO THE HUMAN INTEGRIN ALPHA-6-BETA-4
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DOI：
10.1089/hyb.1990.9.243
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发表时间：
1990-06-01
期刊：
影响因子：
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通讯作者：
SACCHI, A
中科院分区：
文献类型：
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作者：
KENNEL, SJ;EPLER, RG;SACCHI, A
已经制备了针对人整联蛋白α 6 β 4亚基的第二代单克隆抗体。MAb 450-9D、10 D和11 A1在β 4分子上的不同位点反应，MAb（450- 30 A1和33 D）在α 6亚基上的相同位点反应。已经开发了使用这些单克隆抗体组合的双决定簇（双位点）放射免疫测定。β 4的测定区分完整的β 4分子和从C-末端截短的β 4分子（形式c），而另一种测定测量α 6亚基的存在。来自双位点测定的数据支持以下结论：（1）结肠肿瘤和正常结肠粘膜表达大量的α 6 β 4，尽管仅检测到β 4的c型;（2）没有证据表明α 6 β 1在结肠中表达;然而，某些这种复合物可能存在于某些肺肿瘤中。即使β 4亚基的胞质结构域不存在，α 6和β 4的胞外结构域也可以彼此缔合。针对β 4分子的特异性结构域的MAb可用于分析正常和恶性组织中的a型和c型。只有最大的β 4分子“a”保留磷酸化位点的事实可能具有功能意义。
Second generation monoclonal antibodies to the .alpha.6.beta.4 subunits of human integrins have been prepared. MAbs 450-9D, 10D, and 11A1 react at different sites on the .beta.4 molecules and MAbs (450-30A1 and 33D react at the same site on the .alpha.6 subunit. Double determinant (two-site) radioimmunoassays using combinations of these MAbs have been developed. The assays for .beta.4 distinguish between the whole .beta.4 molecule and the .beta.4 molecule truncated from the C-terminus (form c) while another assay measures the presence of .alpha.6 subunits. Data from the two-site assays support the following conclusions: (1) Colon tumors and normal colon mucosa express large amounts of .alpha.6.beta.4 although only form c of the .beta.4 was detected; (2) There is no evidence for .alpha.6.beta.1 expression in colon; however, some of this complex may be present in certain lung tumors. The extracellular domains of .alpha.6 and .beta.4 can associate with each other even if the cytoplasmic domain of the .beta.4 subunit is not present. MAbs to specific domains of the .beta.4 molecule may be useful in analyses of forms a and c in normal and malignant tissue. The fact that only the largest .beta.4 molecule "a" retains the phosphorylation site may have functional significance.