New Directions for Artificial Cells Using Prototyped Biosystems
New Directions for Artificial Cells Using Prototyped Biosystems
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DOI：
10.1021/acs.analchem.8b04885
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发表时间：
2019-04-16
影响因子：
7.4
通讯作者：
Ces, Oscar
中科院分区：
文献类型：
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作者：
Friddin, Mark S.;Elani, Yuval;Ces, Oscar
微流体已经能够产生一系列单室和多室囊泡以及双层描绘的液滴，这些液滴可以在2D和3D中组装。这些模型系统越来越多地被用作人造细胞底盘和仿生结构，用于组装组织模型、工程治疗输送系统和筛选药物。开发该技术的一个瓶颈是设备制造所需的时间、专业知识和设备。这引起了整个微流体学界对使用快速原型设计从计算机辅助设计（CAD）图纸中设计微流体装置的兴趣。我们强调了这种快速原型革命如何改变微流体装置的制造，用于自下而上的合成生物学中的人工细胞构建。我们概述了当前的形势，并介绍了该领域的进步如何产生下一代多功能生物器械，特别是在工业4.0即将到来的情况下。成功开发这项技术并使其开源，可以为新一代公民主导的科学铺平道路，为下一个数十亿美元的初创企业从阁楼或地下室出现的可能性提供动力。
Microfluidics has has enabled the generation of a range of single compartment and multicompartment vesicles and bilayer-delineated droplets that can be assembled in 2D and 3D. These model systems are becoming increasingly used as artificial cell chassis and as biomimetic constructs for assembling tissue models, engineering therapeutic delivery systems, and screening drugs. One bottleneck in developing this technology is the time, expertise, and equipment required for device fabrication. This has led to interest across the microfluidics community in using rapid prototyping to engineer microfluidic devices from computer-aided-design (CAD) drawings. We highlight how this rapid-prototyping revolution is transforming the fabrication of microfluidic devices for artificial cell construction in bottom-up synthetic biology. We provide an outline of the current landscape and present how advances in the field may give rise to the next generation of multifunctional biodevices, particularly with Industry 4.0 on the horizon. Successfully developing this technology and making it open-source could pave the way for a new generation of citizen-led science, fueling the possibility that the next multibillion-dollar start-up could emerge from an attic or a basement.