An ultrastructural examination of the interaction between macrophages and Cryptococcus neoformans
An ultrastructural examination of the interaction between macrophages and Cryptococcus neoformans
复制标题
巨噬细胞和新型隐球菌之间相互作用的超微结构检查
作者：
J. Papadimitriou;T. Robertson;Y. Kletter;M. Aronson;M. Walters
采用超微结构技术在体外和体内研究了单核吞噬细胞与新型隐球菌的相互作用。免疫血清使酵母表面粗糙，在最初的2小时内，增加附着在巨噬细胞表面的生物体数量，以及单个酵母和吞噬细胞之间的接触数量。通过细丝状伪足和细胞质瓣建立接触。在接下来的几天里，巨噬细胞的大小增加，并且，通过它们相邻的细胞表面的紧密附着，细胞屏障包围了现在封闭的酵母。这些事件伴随着真菌荚膜变薄，封闭的隐球菌降解，以及巨噬细胞多核体的形成。过氧化物酶的电子细胞化学技术显示，这些多核细胞主要由刺激的巨噬细胞融合形成。被包围的酵母菌的破坏可能是由于周围细胞分泌各种物质的结果。
The interaction of mononuclear phagocytes with Cryptococcus neoformans was examined in vitro and in vivo using ultrastructural techniques. Immune serum roughens the surface of the yeast and in the first 2 hr, increases the number of organisms attaching to the macrophage surface, as well as the number of contacts between individual yeasts and the phagocyte. Contact is established by means of thin filopodia and cytoplasmic flaps. During the next few days the macrophages increase in size, and, by intimate apposition of their contiguous cell surfaces, a cellular barrier surrounds the now enclosed yeast. These events are accompanied by thinning of fungal capsule, degradation of the enclosed cryptococcus, and the formation of macrophage polykaryons. Electron cytochemical techniques for peroxidase reveal that these multinucleated cells are formed predominantly by the fusion of stimulated macrophages. Destruction of the enclosed yeast probably results from the secretion of various agents by the surrounding cells.