Microbial deposition of gold nanoparticles by the metal-reducing bacterium Shewanella algae
Microbial deposition of gold nanoparticles by the metal-reducing bacterium Shewanella algae
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DOI：
10.1016/j.electacta.2007.02.073
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发表时间：
2007-11-20
影响因子：
6.6
通讯作者：
Nagamine, S.
中科院分区：
文献类型：
--
作者：
Konishi, Y.;Tsukiyama, T.;Nagamine, S.
在pH值为2.0-7.0的条件下，在H-2作为电子供体的存在下，采用嗜中温细菌Shewanella藻类，在25℃条件下实现了金纳米颗粒的微生物还原和沉积。藻静息细胞对金的还原沉积是一个快速的过程，1 mM的AuCl4-离子在30 min内完全还原为单质金。在pH = 7的溶液中，10 ~ 20 nm大小的金纳米颗粒沉积在藻细胞的质周间隙中。当pH值为2.8时，15- 200nm大小的金纳米颗粒沉积在细菌细胞上，这些生物源性纳米颗粒呈现出各种形状，包括纳米三角形：特别是100- 200nm大小的单晶金纳米三角形是微生物沉积的。在pH为2.0的溶液中，约20 nm大小的金纳米颗粒沉积在细胞内，约350 nm大小的金颗粒沉积在细胞外。溶液pH是控制生物成因金颗粒形态和金沉积位置的重要因素。微生物沉积金纳米颗粒作为一种环境友好的替代方法具有潜在的吸引力。(C) 2007 Elsevier Ltd.版权所有。
Microbial reduction and deposition of gold nanoparticles was achieved at 25 degrees C over the pH range 2.0-7.0 using the mesophilic bacterium Shewanella algae in the presence of H-2 as the electron donor. The reductive deposition of gold by the resting cells of S. algae was a fast process: 1 mM AuCl4- ions were completely reduced to elemental gold within 30 min. At a solution pH of 7, gold nanoparticles 10-20 nm in size were deposited in the periplasmic space of S. algae cells. At pH 2.8, gold nanoparticles 15-200 nm in size were deposited on the bacterial cells, and the biogenic nanoparticles exhibited a variety of shapes that included nanotriangles: in particular, single crystalline gold nanotriangles 100-200 nm in size were microbially deposited. At a solution pH of 2.0, gold nanoparticles about 20 nm in size were deposited intracellularly, and larger gold particles approximately 350 nm in size were deposited extracellularly. The solution pH was an important factor in controlling the morphology of the biogenic gold particles and the location of gold deposition. Microbial deposition of gold nanoparticles is potentially attractive as an environmentally friendly alternative to conventional methods. (C) 2007 Elsevier Ltd. All rights reserved.