Genetic dissection of complex traits with chromosome substitution strains of mice
Genetic dissection of complex traits with chromosome substitution strains of mice
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DOI：
10.1126/science.1093139
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发表时间：
2004-04-16
期刊：
影响因子：
56.9
通讯作者：
Nadeau, JH
中科院分区：
文献类型：
--
作者：
Singer, JB;Hill, AE;Nadeau, JH
染色体替代菌株（CSSs）已被提出作为一种简单而有效的方法来鉴定影响发育、生理和行为过程的数量性状位点（qtl）。在这里，我们报道了一个脊椎动物物种的完整CSS面板的构建。CSS面板由22个小鼠品系组成，每个品系携带一条从供体品系（a /J）替换到共同宿主背景（C57BL/6J）上的染色体。一项对53个性状的调查揭示了150个qtl影响血清固醇和氨基酸水平、饮食引起的肥胖和焦虑的证据。这些结果表明，css极大地促进了A/J和C57BL/6J自交系中控制自然表型变异多样性的基因的检测和鉴定。
Chromosome substitution strains (CSSs) have been proposed as a simple and powerful way to identify quantitative trait loci (QTLs) affecting developmental, physiological, and behavioral processes. Here, we report the construction of a complete CSS panel for a vertebrate species. The CSS panel consists of 22 mouse strains, each of which carries a single chromosome substituted from a donor strain (A/J) onto a common host background (C57BL/6J). A survey of 53 traits revealed evidence for 150 QTLs affecting serum levels of sterols and amino acids, diet-induced obesity, and anxiety. These results demonstrate that CSSs greatly facilitate the detection and identification of genes that control the wide diversity of naturally occurring phenotypic variation in the A/J and C57BL/6J inbred strains.