Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans
Live-cell and super-resolution imaging reveal that the distribution of wall-associated protein A is correlated with the cell chain integrity of Streptococcus mutans
复制标题
活细胞和超分辨率成像揭示壁相关蛋白 A 的分布与变形链球菌的细胞链完整性相关
DOI：
10.1111/omi.12100
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发表时间：
2015-10-01
影响因子：
3.7
通讯作者：
Sun, Y.
中科院分区：
文献类型：
--
作者：
Li, Y.;Liu, Z.;Sun, Y.
变形链球菌是引起龋病的主要致病菌。它具有出色的形成生物膜的能力，这对毒力至关重要。以往的研究表明，WAPA基因的敲除会影响变形链球菌的细胞链和生物膜的形成。作为一种表面蛋白，WAPA的分布尚不清楚，但了解WAPA的功能机制很重要。本研究以荧光蛋白mCherry为报告基因，通过时间推移和超分辨荧光成像技术研究了WAPA在变形链球菌中的动态分布。结果揭示了WAPA在变形链球菌单链、分裂链和长链中有趣的亚细胞分布模式。它出现在细胞的中央，并随着细胞的生长和分裂而移动到两极。在细胞链中，在每一轮细胞分裂后，这种动态重新定位导致WAPA在之前的细胞分裂位点上的分布，从而导致WAPA位于两个相邻细胞对的边界上的模式。这种WAPA分布模式对应于WAPA缺失细胞链的断裂分段。WAPA在细胞周期中的动态定位增加了我们对WAPA在维持细胞链完整性和生物膜形成方面的机制的理解。
Streptococcus mutans is a primary pathogen responsible for dental caries. It has an outstanding ability to form biofilm, which is vital for virulence. Previous studies have shown that knockout of Wall-associated protein A (WapA) affects cell chain and biofilm formation of S.mutans. As a surface protein, the distribution of WapA remains unknown, but it is important to understand the mechanism underlying the function of WapA. This study applied the fluorescence protein mCherry as a reporter gene to characterize the dynamic distribution of WapA in S.mutans via time-lapse and super-resolution fluorescence imaging. The results revealed interesting subcellular distribution patterns of WapA in single, dividing and long chains of S.mutans cells. It appears at the middle of the cell and moves to the poles as the cell grows and divides. In a cell chain, after each round of cell division, such dynamic relocation results in WapA distribution at the previous cell division sites, resulting in a pattern where WapA is located at the boundary of two adjacent cell pairs. This WapA distribution pattern corresponds to the breaking segmentation of wapA deletion cell chains. The dynamic relocation of WapA through the cell cycle increases our understanding of the mechanism of WapA in maintaining cell chain integrity and biofilm formation.