A nucleic acid probe labeled with desmethyl thiazole orange : A new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging
A nucleic acid probe labeled with desmethyl thiazole orange : A new type of hybridization-sensitive fluorescent oligonucleotide for live-cell RNA imaging
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去甲基噻唑橙标记的核酸探针：一种用于活细胞 RNA 成像的新型杂交敏感荧光寡核苷酸
DOI：
10.1039/c2ob26707a
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
D. O
中科院分区：
文献类型：
--
作者：
Okamoto;A. ; Sugizaki;K. ; Yuki;M. ; Yanagisawa;H. ; Ikeda;S. ; Sueoka;T. ; Hayashi;G. ; Wang;D. O
一种新的去甲基噻唑橙子染料荧光核苷酸D′505已被开发出来，用于扩大活细胞RNA成像荧光探针的功能。D′505的核苷单元可溶于有机溶剂，这使得核苷单元的制备和DNA合成循环中的反应更加高效。含D′505的寡核苷酸染料在pH 7以下质子化，通过控制D′505染料的激子相互作用，寡核苷酸显示杂交敏感的荧光发射。简化的程序和有效的杂交敏感的荧光发射产生多色杂交敏感的荧光探针，这是有用的活细胞RNA成像。受体漂白法为我们提供了许多活细胞中特定细胞中RNA的信息。
A new fluorescent nucleotide with desmethyl thiazole orange dyes, D′505, has been developed for expansion of the function of fluorescent probes for live-cell RNA imaging. The nucleoside unit of D′505 for DNA autosynthesis was soluble in organic solvents, which made the preparation of nucleoside units and the reactions in the cycles of DNA synthesis more efficient. The dyes of D′505-containing oligodeoxynucleotide were protonated below pH 7 and the oligodeoxynucleotide exhibited hybridization-sensitive fluorescence emission through the control of excitonic interactions of the dyes of D′505. The simplified procedure and effective hybridization-sensitive fluorescence emission produced multicolored hybridization-sensitive fluorescent probes, which were useful for live-cell RNA imaging. The acceptor-bleaching method gave us information on RNA in a specific cell among many living cells.