Design rules of synthetic non-coding RNAs in bacteria
Design rules of synthetic non-coding RNAs in bacteria
复制标题
DOI：
10.1016/j.ymeth.2018.01.001
复制
发表时间：
2018-07-01
期刊：
影响因子：
4.8
通讯作者：
Moon, Tae Seok
中科院分区：
文献类型：
--
作者：
Lee, Young Je;Moon, Tae Seok
合成生物学的长期目标之一是开发具有可预测行为的可设计遗传部分，可用于实现不同的细胞功能。非编码 RNA 的发现及其在细胞加工中的重要性迅速吸引了研究人员对设计功能性非编码 RNA 分子的关注。这些合成的非编码 RNA 具有受 Watson-Crick 碱基配对控制的简单设计原理，但表现出越来越复杂的功能。重要的是，由于其特定和模块化的行为，合成非编码RNA已被广泛用于调节靶基因的转录和翻译。在这篇综述中，我们总结了用于工程合成非编码 RNA 的各种设计规则和策略。具体来说，我们讨论了如何将 RNA 分子转化为强大的调节因子并用于控制靶基因表达。随着可推广的非编码RNA设计规则的建立，研究界将把重点从蛋白质调节剂转向RNA调节剂。 (C) 2018 Elsevier Inc. 保留所有权利。
One of the long-term goals of synthetic biology is to develop designable genetic parts with predictable behaviors that can be utilized to implement diverse cellular functions. The discovery of non-coding RNAs and their importance in cellular processing have rapidly attracted researchers' attention towards designing functional non-coding RNA molecules. These synthetic non-coding RNAs have simple design principles governed by Watson-Crick base pairing, but exhibit increasingly complex functions. Importantly, due to their specific and modular behaviors, synthetic non-coding RNAs have been widely adopted to modulate transcription and translation of target genes. In this review, we summarize various design rules and strategies employed to engineer synthetic non-coding RNAs. Specifically, we discuss how RNA molecules can be transformed into powerful regulators and utilized to control target gene expression. With the establishment of generalizable non-coding RNA design rules, the research community will shift its focus to RNA regulators from protein regulators. (C) 2018 Elsevier Inc. All rights reserved.