Correlation of CENP-F gene expression with tumor-proliferating activity in human salivary gland tumors
Correlation of CENP-F gene expression with tumor-proliferating activity in human salivary gland tumors
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DOI：
10.1016/j.oraloncology.2005.03.008
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发表时间：
2005-08-01
期刊：
影响因子：
4.8
通讯作者：
Kamata, N
中科院分区：
文献类型：
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作者：
Shigeishi, H;Mizuta, K;Kamata, N
采用实时定量逆转录聚合酶链反应（RT-PCR）检测了26例人涎腺肿瘤（7例多形性腺瘤、3例Warthin瘤、7例粘液表皮样癌、4例腺样囊性癌、4例腺泡细胞癌和1例恶性肌上皮瘤）和4例正常颌下腺中着丝粒蛋白F (CENP-F) mRNA的表达。恶性肿瘤组织中CENP-F mRNA的平均表达量（1.05 +/- 0.32）高于正常颌下腺组织（0.11 +/- 0.05）和良性肿瘤组织（0.46 +/- 0.16）。我们发现16例恶性肿瘤中CENP-F mRNA表达水平与临床分期有显著相关性（Mann-Whitney U检验，p = 0.027）。我们还发现Ki-67标记指数与恶性肿瘤中CENP-F的表达有显著相关性（Spearmans相关系数经秩次检验，p = 0.029）。这些结果表明，人类CENP-F mRNA与恶性肿瘤中细胞增殖的增加或异常密切相关。(c) 2005 Elsevier Ltd版权所有。
We examined the expression of Centromere protein F (CENP-F) mRNA in 26 human salivary gland tumors (seven pleomorphic adenomas, three Warthin tumors, seven mucoepidermoid carcinomas, four adenoid cystic carcinomas, four acinic cell carcinomas and one malignant myoepithelioma) and four normal submandibular glands using the real time quantitative reverse transcription-polymerase chain reaction (RT-PCR). The mean expression level of CENP-F mRNA was higher in malignant tumors (1.05 +/- 0.32) than normal submandibular glands (0.11 +/- 0.05) and benign tumors (0.46 +/- 0.16). We found a significant association between the level of CENP-F mRNA expression and clinical stage in 16 malignant tumors (Mann-Whitney U test, p = 0.027). We also found a significant correlation between the Ki-67 labeling index and CENP-F expression in malignant tumors (Spearmans correlation coefficient by rank test, p = 0.029). These results indicate that human CENP-F mRNA is closely linked to the increased or abnormal cell proliferation in malignant conditions. (c) 2005 Elsevier Ltd. All rights reserved.