A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus
A nested set of six or seven subgenomic mRNAs is formed in cells infected with different isolates of porcine reproductive and respiratory syndrome virus
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DOI：
10.1099/0022-1317-77-6-1265
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发表时间：
1996-06-01
影响因子：
3.8
通讯作者：
Halbur, PG
中科院分区：
文献类型：
--
作者：
Meng, XJ;Paul, PS;Halbur, PG
对猪繁殖与呼吸综合征病毒 (PRRSV) 的亚基因组 mRNA (sg mRNA) 进行了表征。在PRRSV感染的细胞中形成3'共末端嵌套组的sg mRNA的数量在具有不同毒力的PRRSV分离株中从6到7个不等。 PRRSV 一些分离株中的其他种类的 sg mRNA 被指定为 sg mRNA 3-1。发现 sg mRNA 3、3-1 和 4 的前导 mRNA 连接序列含有相似的六核苷酸序列基序 (U(G)UA(G/C)ACC。通过将 sg mRNA 3-1 的 5' 末端序列与两个分离株 ISU79 和 ISU1894 的基因组序列进行比较，发现从分离株 ISU1894 中的 U 到分离株 ISU79 中的 C 的点突变，导致与在分离株 ISU79 中获得新的前导 mRNA 连接序列 (UUGACC) 相关，因此在 sg mRNA 3-1 的 5' 端鉴定出另外一种 sg mRNA 3-1。
The subgenomic mRNAs (sg mRNA) of procine reproductive and respiratory syndrome virus (PRRSV) were characterized. The number of sg mRNAs, which form a 3'-coterminal nested set in PRRSV-infected cells, varied from six to seven among PRRSV isolates with differing virulence. The additional species of sg mRNA in some isolates of PRRSV was designated as sg mRNA 3-1. The leader-mRNA junction sequences of sg mRNAs 3, 3-1 and 4 were found to contain a similar six nucleotide sequence motif, (U(G)UA(G/C)ACC. By comparing the 5'-terminal sequence of sg mRNA 3-1 with the genomic sequence of two isolates, ISU79, and ISU1894, it was found that a point mutation, from U in isolate ISU1894 to C in isolate ISU79, led to the acquisition of a new leader-mRNA junction sequence (UUGACC) in isolate ISU79, and therefore an additional species of sg mRNA 3-1. A small ORF (3-1) was identified at the 5' end of sg mRNA 3-1.