Adsorption of Proteins to Thin-Films of PDMS and Its Effect on the Adhesion of Human Endothelial Cells.
Adsorption of Proteins to Thin-Films of PDMS and Its Effect on the Adhesion of Human Endothelial Cells.
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DOI：
10.1039/c1ra00198a
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发表时间：
2011-09-21
期刊：
影响因子：
3.9
通讯作者：
Garcia CD
中科院分区：
文献类型：
--
作者：
Chumbimuni-Torres KY;Coronado RE;Mfuh AM;Castro-Guerrero C;Silva MF;Negrete GR;Bizios R;Garcia CD
本文介绍了一种简单、廉价的聚二甲基硅氧烷薄膜的制备方法。通过多种技术（椭圆偏振法、核磁共振、原子力显微镜和测角法）对此类薄膜进行了表征，并用于研究不同条件下三种模型蛋白质（纤维蛋白原、I型胶原和牛血清白蛋白）的吸附动力学。从蛋白质吸附研究中收集的信息，然后被用来研究人真皮微血管内皮细胞的粘附。这些研究的结果表明，这些薄膜可以用来模拟与商业PDMS制造的微器件的表面特性。此外，该论文还提供了在BioMEMS设备中有效附着细胞的指导方针。
This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.