Succession and Phylogenetic Profile of Eubacterial Communities in Rice Straw Incorporated into a Rice Field: Estimation by PCR-DGGE Analysis
Succession and Phylogenetic Profile of Eubacterial Communities in Rice Straw Incorporated into a Rice Field: Estimation by PCR-DGGE Analysis
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DOI：
10.1111/j.1747-0765.2005.tb00006.x
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发表时间：
2005-02
影响因子：
2
通讯作者：
Atsuo Sugano;Hidetaka Tsuchimoto;Cho Cho Tun-Cho;S. Asakawa;M. Kimura
中科院分区：
文献类型：
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作者：
Atsuo Sugano;Hidetaka Tsuchimoto;Cho Cho Tun-Cho;S. Asakawa;M. Kimura
采用PCR-DGGE分析和测序方法，对稻田秸秆分解菌群落的演替和系统发育特征进行了分析。叶鞘和叶片部分分别装入尼龙网袋中，在退耕季排水条件下和水稻移栽后淹水条件下置于稻田土壤中。此外，在水稻移栽后，在旱作季排水条件下放置的RS样品再次放置在淹水稻田土壤中。RS样品中细菌群落的DGGE模式可分为叶鞘和叶片两类。主成分分析揭示了群落演替随放置时间的延长而变化。这些结果表明，RS部分（鞘或叶片）主要决定了负责RS分解的细菌群落的结构，其次是放置时间。特异DGGE条带的序列分析表明，与这些条带有亲缘关系的大部分属于α-、β-、γ-和δ-变形菌门、CFB组和螺旋体菌纲。部分条带与酸杆菌和Verrucomicrobia密切相关。CFB成员和α-Proteobacteria在RS部位普遍占优势，而γ-和δ-Proteobacteria以及Spirochaetes和β-Proteobacteria分别在鞘和叶片部位特异定植。此外，Proteobacteria和CFB成员在淹水或排水条件下描述了细菌群落的差异。综上所述，在淹水和排水条件下，稻田土壤中RS的分解主要由变形菌、CFB菌群和螺旋体菌负责。
PCR-DGGE analysis followed by sequencing was conducted to estimate the succession and the phylogenetic profile of the eubacterial communities responsible for the decomposition of rice straw (RS) that was incorporated into a rice field. Leaf sheath and leaf blade parts were separately put in nylon mesh bags, and were placed in the rice field soil under drained conditions during the off-cropping season and under flooded conditions after transplantation of rice. In addition, RS samples that had been placed under drained conditions in the off-cropping season were placed again in flooded rice field soil after transplantation of rice. DGGE patterns of the bacterial communities in the RS samples were classified into two groups, namely leaf sheaths and leaf blades. Principal component analysis of the DGGE patterns revealed the succession along with the duration of placement. These results indicated that the RS part (sheath or blade) mainly determined the structure of the bacterial communities responsible for the RS decomposition, followed by the duration of placement. Sequence analysis of the characteristic DGGE bands indicated that most of the closest relatives associated with the bands belonged to α-, β-, γ-, and δ-Proteobacteria, CFB group, and Spirochaetes. Some bands were closely related to Acidobacteria and Verrucomicrobia. CFB members and α-Proteobacteria predominated commonly in both RS parts, while γ- and δ-Proteobacteria, and Spirochaetes and β-Proteobacteria specifically colonized sheath and blade parts, respectively. In addition, Proteobacteria and CFB members characterized the differences in the bacterial communities under flooded or drained conditions. These results suggest that Proteobacteria, CFB group, and Spirochaetes were responsible for RS decomposition in rice field soil under both flooded and drained conditions.