A NEW FACTOR RELATED TO TATA-BINDING PROTEIN HAS HIGHLY RESTRICTED EXPRESSION PATTERNS IN DROSOPHILA
A NEW FACTOR RELATED TO TATA-BINDING PROTEIN HAS HIGHLY RESTRICTED EXPRESSION PATTERNS IN DROSOPHILA
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DOI：
10.1038/361557a0
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发表时间：
1993-02-11
期刊：
影响因子：
64.8
通讯作者：
TJIAN, R
中科院分区：
文献类型：
--
作者：
CROWLEY, TE;HOEY, T;TJIAN, R
TATA结合蛋白TBP是真核基因转录所必需的。TBP与不同的TBP相关因子形成的多蛋白复合体参与了聚合酶I和II的转录启动，也可能是IL1。在活性起始复合体的形成过程中，TBP与其他蛋白质进行特定的接触，例如TFIIB和RNA聚合酶II(参考文献2-4)。在这里，我们描述了果蝇基因产物的克隆和鉴定，该基因产物与TBP具有相当大的序列相似性，并且在胚胎中具有高度限制性的表达模式。这种TBP相关因子是一种DNA结合蛋白，但不太可能是基础转录因子。我们的结果表明，TBP相关因子是一种序列特异性转录因子，具有TBP的DNA结合特性。
THE TATA-binding protein TBP is necessary for the transcription of eukaryotic genes. Multi-protein complexes formed by TBP and different TBP-associated factors are involved in the initiation of transcription by polymerases I and II, and probably ill as well1. During the formation of an active initiation complex, TBP makes specific contacts with other proteins, for example TFIIB and RNA polymerase II (refs 2-4). Here we describe the cloning and characterization of a Drosophila gene product with considerable sequence similarity to TBP and a highly restricted expression pattern in the embryo. This TBP-related factor is a DNA-binding protein but is not likely to be a basal transcription factor. Our results suggest that TBP-related factor is a sequence-specific transcription factor that shares the DNA-binding properties of TBP.