Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters
Characterization of synovial mast cells in knee osteoarthritis: association with clinical parameters
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DOI：
10.1016/j.joca.2015.11.011
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发表时间：
2016-04-01
影响因子：
7
通讯作者：
Ioan-Facsinay, A.
中科院分区：
文献类型：
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作者：
de Lange-Brokaar, B. J. E.;Kloppenburg, M.;Ioan-Facsinay, A.
目的：以类风湿关节炎(RA)为对照，探讨肥大细胞在骨性关节炎(OA)滑膜中的表达及其与临床参数的关系。2~3张石蜡切片用苏木精-伊红(H&E)染色(滑膜评分0-9)定量炎症反应，用CD117和类胰蛋白酶双重免疫荧光定量肥大细胞数(每10个高倍视野)。每例患者的平均得分用于分析。根据Kellgren and Lawrence(KL)膝关节X线片评分标准对患者膝关节X线片进行评分，并对患者的疼痛程度进行视觉模拟评分。结果：与RA样本4(1-47)相比，OA样本45(1-168)的肥大细胞的中位数(范围)显著增加(P<0.001)，而滑膜炎症评分的中位数(范围)较低(0-6.0)。滑膜炎症积分与肥大细胞数显著相关(OASpearman‘s Rho(P值)0.023，RA0.5(P值0.001))。有趣的是，我们观察到了一种趋势，即肥大细胞的数量与独立于滑膜炎症的骨性关节炎患者的KL分级增加有关(P值0.05)。结论：肥大细胞在骨性关节炎患者滑膜组织中的发生率较高，并与结构损伤有关，提示肥大细胞在该病中起一定作用。(C)2015年国际骨性关节炎研究会。爱思唯尔有限公司出版。保留所有权利。
Objective: To investigate the presence of mast cells in the osteoarthritic (OA) synovium and their association with clinical parameters in comparison with rheumatoid arthritis (RA) samples.Method: Synovial tissues of 56 symptomatic OA and 49 RA patients were obtained. Two to three paraffin slides were used to quantify inflammation using haematoxylin and eosin (H&E) staining (synovitis score 0-9), and numbers of mast cells (per 10 high-power fields) using double immunofluorescence for CD117 and tryptase. Average scores per patient were used for analysis. Knee radiographs of OA patients were scored according to the Kellgren and Lawrence (KL) system and pain was determined in OA patients at baseline by visual analogue scale (VAS).Results: Median (range) of mast cells was significantly higher in OA samples 45 (1-168) compared to RA samples 4 (1-47) (P-value < 0.001), despite a lower median (range) synovitis score in OA (2.5 (0-6.0)) compared to 4.6 (0-8.0) in RA samples. The synovitis score was significantly correlated with the number of mast cells (in OA Spearman's rho (P-value) 0.3 (0.023) and RA 0.5 (P-value < 0.001)). Interestingly, we observed a trend towards an association between the number of mast cells and an increased KL-grade (P-value 0.05) in OA patients, independently of synovitis. No associations were found with self-reported pain.Conclusion: Prevalence of mast cells in OA synovial tissue is relatively high and associates with structural damage in OA patients, suggesting a role of mast cells in this disease. (C) 2015 Osteoarthritis Research Society International. Published by Elsevier Ltd. All rights reserved.