Dose--response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia.
Dose--response curve for ethylnitrosourea-induced specific-locus mutations in mouse spermatogonia.
复制标题
乙基亚硝基脲诱导的小鼠精原细胞特异性位点突变的剂量反应曲线。
DOI：
10.1073/pnas.79.11.3589
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发表时间：
1982
影响因子：
11.1
通讯作者：
Thompson,HM
中科院分区：
文献类型：
--
作者：
Russell,WL;Hunsicker,PR;Raymer,GD;Steele,MH;Stelzner,KF;Thompson,HM
N-乙基-N-亚硝基脲在小鼠中的极端致突变效果允许积累迄今为止获得的化学诱导精原细胞特定位点突变的最广泛的剂量反应数据。在曲线的下部，低于100 mg/kg的剂量，数据在统计学上显著低于拟合直线的最大似然。独立证据表明，在此剂量范围内，乙基亚硝基脲到达睾丸的量与注射剂量成正比。它的结论是，尽管乙基亚硝基脲的致突变效果，精原细胞显然能够修复至少一个主要部分的突变损伤时，修复过程中没有淹没高剂量。这一发现对化学品致突变作用的基础研究和风险评估都具有重要意义。
The extreme mutagenic effectiveness of N-ethyl-N-nitrosourea in the mouse has permitted the accumulation of the most extensive dose--response data yet obtained for chemical induction of specific-locus mutations in spermatogonia. In the lower portion of the curve, below a dose of 100 mg/kg, the data fall statistically significantly below a maximum likelihood fit to a straight line. Independent evidence indicates that, over this dose range, ethylnitrosourea reaches the testis in amounts directly proportional to the injected dose. It is concluded that, despite the mutagenic effectiveness of ethylnitrosourea, the spermatogonia are apparently capable of repairing at least a major part of the mutational damage when the repair process is not swamped by a high dose. This finding is important both in basic studied on the mutagenic action of chemicals in mammals and in risk estimation.