The role of subcellular localization in initiation of apoptosis by photodynamic therapy
The role of subcellular localization in initiation of apoptosis by photodynamic therapy
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DOI：
10.1111/j.1751-1097.1997.tb08581.x
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发表时间：
1997-03-01
影响因子：
3.3
通讯作者：
Chang, CK
中科院分区：
文献类型：
--
作者：
Kessel, D;Luo, Y;Chang, CK
光动力疗法可诱导细胞迅速凋亡，这取决于所用的细胞系和光敏剂。本研究以培养的P388小鼠白血病细胞系为材料，评价了两种结构相关的光敏剂的光动力效应。PDT后60 min内，A类药物八乙基紫嘌呤脒锡、靶向溶酶体、线粒体和细胞膜均可见凋亡细胞核;直到PDT后24小时才观察到凋亡细胞核，这些结果与最近的其他报告一起，与膜光损伤可以延迟或阻止PDT引起的凋亡反应的假设一致。
Rapid initiation of apoptosis can be induced by photodynamic therapy, depending on the cell line and sensitizer employed, In this study, we evaluated the photodynamic responses to two structurally related photosensitizing agents, using the P388 murine leukemia cell line in culture, Photodamage mediated by tin etiopurpurin involved lysosomes and mitochondria and yielded a rapid apoptotic response; apoptotic nuclei were observed within 60 min after PDT, A drug analog, tin octaethylpurpurin amidine, targeted lysosomes, mitochondria and cell membranes; apoptotic nuclei were not observed until 24 h after PDT, These results, together with other recent reports, are consistent with the hypothesis that membrane photodamage can delay or prevent an apoptotic response to PDT.