Endurance performance in well-trained mice is enhanced by short-duration intermittent hypoxia via improved muscle fatty acid metabolism
Endurance performance in well-trained mice is enhanced by short-duration intermittent hypoxia via improved muscle fatty acid metabolism
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通过改善肌肉脂肪酸代谢，短时间间歇性缺氧可以增强训练有素的小鼠的耐力表现
DOI：
10.1113/ep086254
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发表时间：
2017
影响因子：
2.7
通讯作者：
Suzuki J
中科院分区：
文献类型：
--
作者：
Suzuki J
作者先前报道，短时间间歇性缺氧通过增强脂肪酸代谢对耐力的改善具有累加效应。本研究旨在探讨短期间歇性低氧对训练小鼠耐力的影响。训练组小鼠从5周龄起在装有轮子活动装置的笼子中饲养7周。自愿跑步显著增加了最大运动能力（5.9倍）。训练后的小鼠进行常氧跑台训练（Tr）或短时间歇低氧训练（12%O2 15 min，20.9%O2 10 min，4次，IntTr）4周。IntTr组的最大运动能力显著高于Tr组（36%）。总肉毒碱棕榈酰转移酶活性在比目鱼肌和跖肌中显着大于IntTr（分别为13%和22%）比Tr。间歇性低氧训练显著增加了跖肌和红腓肠肌中I型纤维的比例。过氧化物酶体增殖物激活受体γ共激活因子1-α在核中的表达在IntTr的比目鱼肌（5.2倍）和红腓肠肌（3.1倍）中显著高于Tr。而FAT/CD 36蛋白表达显着增加训练后，有和无间歇性缺氧的红腓肠肌，其表达水平显着高于（21%）在IntTr比Tr。目前的研究结果表明，运动训练在常氧气氛下与短期间歇性缺氧是一个有用的策略，以提高耐力的表现，在高度训练的啮齿动物。
The author previously reported that short-duration intermittent hypoxia had additive effects on improvements in endurance capacity by enhancing fatty acid metabolism. The present study was designed to investigate the effects of short-duration intermittent hypoxia on endurance capacity in well-trained mice. Mice in the training group were housed in a cage with a wheel activity device for 7 weeks from 5 weeks old. Voluntary running markedly increased maximal exercise capacity (by 5.9-fold). Trained mice were then subjected to either normoxic treadmill training (Tr) or training with short-duration intermittent hypoxia (12% O2 for 15 min, 20.9% O2 for 10 min, 4 times, IntTr) for 4 weeks. Maximal exercise capacity was significantly greater in IntTr (by 36%) than in Tr. Total carnitine palmitoyl transferase activity in the soleus and plantaris muscles was significantly greater in IntTr (by 13% and 22%, respectively) than in Tr. Training with intermittent hypoxia significantly increased the proportion of type I fibres in the plantaris and red gastrocnemius muscles. Peroxisome proliferator-activated receptor gamma coactivator 1-alpha expression in the nucleus was significantly greater in the soleus (5.2-fold) and red gastrocnemius (3.1-fold) muscles in IntTr than in Tr. While FAT/CD36 protein expression significantly increased after training with and without intermittent hypoxia in the red gastrocnemius muscle, its expression levels were markedly higher (by 21%) in IntTr than in Tr. The present results suggest that exercise training under normoxic atmosphere with short-duration intermittent hypoxia represents a useful strategy for improving endurance performance in highly trained rodents.