Proteomic analysis of preterm premature rupture of membranes in placental tissue
Proteomic analysis of preterm premature rupture of membranes in placental tissue
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DOI：
10.1007/s00404-013-2837-5
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发表时间：
2013-10-01
影响因子：
2.6
通讯作者：
Wang, Peng
中科院分区：
文献类型：
--
作者：
Chang, Aimin;Zhang, Zhan;Wang, Peng
胎膜早破 (PPROM) 是一种产科并发症，是围产期死亡率和发病率的主要原因。 PPROM 会导致重症监护紧急情况，并且几乎所有 PPROM 事件都是自发的且不可预测。此外，妊娠期间胎盘组织中蛋白质组的变化导致未胎膜早破尚不清楚。我们利用蛋白质组学方法来研究人类未胎膜早破背后的分子机制。更好地了解蛋白质组改变可能有助于识别更好的诊断/预后标记。在明显区分的未足月胎膜早破病例和健康足月对照中收集人胎盘组织。利用二维凝胶聚丙烯酰胺电泳结合质谱和生物信息学分析来鉴定表达改变的蛋白质。在本研究中，仅选择最重要的蛋白质差异进行进一步分析。大多数已鉴定的蛋白质是细胞的结构/细胞骨架成分，或参与能量代谢和氧化应激的调节。因此，这种方法导致了几种参与潜在病理生理机制的蛋白质的鉴定，这些蛋白质可以进一步作为新的诊断工具和合理药物干预的目标。
Preterm premature rupture of membranes (PPROM) is an obstetrics complication and is the leading cause of perinatal mortality and morbidity. PPROM results in critical care emergencies, and nearly all PPROM events are spontaneous and unpredictable. In addition, changes in the proteome in placental tissue during pregnancy that lead to PPROM are not clear.We utilize a proteomics approach to study the molecular mechanisms behind human PPROM. A better understanding of proteome alteration could lead to the identification of better diagnostic/prognostic markers. Human placental tissue was collected in clearly differentiated cases of PPROM and in a healthy term control. Two-dimensional gel polyacrylamide electrophoresis coupled with mass spectrometry and bioinformatics analysis was utilized to identify proteins with altered expression.In this study, only the most important protein differences were selected for further analysis. Most of the identified proteins were structural/cytoskeletal components of the cell or involved in the regulation of energy metabolism and oxidative stress.As a result, this approach has led to the identification of several proteins involved in the underlying pathophysiological mechanisms that can further serve as novel diagnostic tools and targets for rational drug intervention.