Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos.
Live Imaging of Epithelial-Mesenchymal Transition in Mesoderm Cells of Gastrulating Drosophila Embryos.
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DOI：
10.1007/978-1-0716-0779-4_8
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发表时间：
2021
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上皮-间充质转化（EMT）在进化和发育过程中驱动细胞多样性的产生。越来越多的证据表明，EMT不是一个二元开关，而是一个可逆的过程，可以稳定在中间状态。尽管我们对触发EMT的信号通路有着广泛的了解，但我们对EMT如何逐步发生知之甚少。在完整的活体动物中进行EMT的细胞的实时成像将为我们提供关于EMT如何在细胞和分子水平上执行的有价值的见解，并帮助我们识别和理解中间状态。在这里，我们描述了如何成像早期阶段的EMT活果蝇胚胎中胚层细胞和如何图像收缩肌球蛋白，暂停EMT进展。
Epithelial-mesenchymal transitions (EMTs) drive the generation of cell diversity during both evolution and development. More and more evidence has pointed to a model where EMT is not a binary switch but a reversible process that can be stabilized at intermediate states. Despite our vast knowledge on the signaling pathways that trigger EMT, we know very little about how EMT happens in a step-wise manner. Live imaging of cells that are undergoing EMT in intact, living, animals will provide us valuable insights into how EMT is executed at both the cellular and molecular levels and help us identify and understand the intermediate states. Here, we describe how to image early stages of EMT in the mesoderm cells of live Drosophila melanogaster embryos and how to image contractile myosin that suspends EMT progression.