Adiponectin accelerates reverse cholesterol transport by increasing high density lipoprotein assembly in the liver
Adiponectin accelerates reverse cholesterol transport by increasing high density lipoprotein assembly in the liver
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DOI：
10.1016/j.bbrc.2007.05.040
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发表时间：
2007-07-13
影响因子：
3.1
通讯作者：
Yamashita, Shizuya
中科院分区：
文献类型：
--
作者：
Matsuura, Fumihiko;Oku, Hiroyuki;Yamashita, Shizuya
血浆高密度脂蛋白(HDL)-胆固醇水平与冠状动脉疾病的发病率呈负相关。高密度脂蛋白在防止动脉粥样硬化中起着重要的作用，它通过清除动脉粥样硬化中的胆固醇并将其运送回肝脏。atp结合盒转运体（ABCA1和ABCG1）和清道夫受体BI （SR-BI）被认为是肝脏中生成HDL的限速因素之一。脂肪细胞分泌的脂联素（APN）也是抑制动脉粥样硬化发展的重要分子之一。最近有报道称，人血浆高密度脂蛋白胆固醇水平与血浆APN浓度呈正相关。因此，我们研究了APN与肝脏中HDL组装的关系。将人肝癌细胞系HepG2细胞在指定浓度的重组APN培养基中培养24 h。APN可提高HepG2细胞载脂蛋白A-I (apoA-I) mRNA水平，增加apoA-I从细胞向培养基的分泌。此外，APN增加了HepG2细胞中ABCA 1的mRNA和蛋白水平，但没有增加ABCG1和SR-BI的水平。综上所述，目前的研究表明，APN可能通过增强肝脏中ABCA1途径和apoA-I合成来增加HDL组装，从而预防动脉粥样硬化。(C) 2007爱思唯尔公司版权所有。
Plasma high density lipoprotein (HDL)-cholesterol levels are negatively correlated with the incidence of coronary artery disease. HDL plays an important role in protecting against atherosclerosis by removing cholesterol from atheroma and transporting it back to the liver. The ATP-binding cassette transporters (ABCA1 and ABCG1) and scavenger receptor BI (SR-BI) are thought to be one of the rate-limiting factors to generate HDL in the liver. Adiponectin (APN) secreted from adipocytes is also one of the important molecules to inhibit the development of atherosclerosis. Recently, it has been reported that plasma HDL-cholesterol levels are positively correlated with plasma APN concentrations in humans. Therefore, we investigated the association of APN with HDL assembly in the liver. Human hepatoma cell line, HepG2 cells, were incubated for 24 h in the culture medium with the indicated concentrations of recombinant APN. APN enhanced the mRNA level of apolipoprotein A-I (apoA-I) in HepG2 cells and increased the secretion of apoA-I from the cells to the medium. Furthermore, APN increased both mRNA and protein levels of ABCA 1, but not ABCG1 and SR-BI, in HepG2 cells. Taken together, the current study demonstrates that APN might protect against atherosclerosis by increasing HDL assembly through enhancing ABCA1 pathway and apoA-I synthesis in the liver. (C) 2007 Elsevier Inc. All rights reserved.