Cytoplasmic gene expression system enhances the efficiency of cationic liposome-mediated in vivo gene transfer into mouse brain.
Cytoplasmic gene expression system enhances the efficiency of cationic liposome-mediated in vivo gene transfer into mouse brain.
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细胞质基因表达系统提高了阳离子脂质体介导的体内基因转移至小鼠大脑的效率。
DOI：
10.1006/bbrc.1997.6568
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发表时间：
1997
影响因子：
3.1
通讯作者：
T. Mayumi
中科院分区：
文献类型：
--
作者：
H. Mizuguchi;T. Nakagawa;Y. Morioka;S. Imazu;M. Nakanishi;T. Kondo;T. Hayakawa;T. Mayumi
中枢神经系统（CNS）基因转移方法的发展是重要的基础研究以及基因治疗的临床研究。阳离子脂质体（CL）由于其安全性和易用性而成为有吸引力的载体。然而，迄今为止，只有低成功率的报告。我们成功地获得了高转染效率的新生小鼠脑内的CL和细胞质基因表达系统的基础上T7 RNA聚合酶和T7 RNA聚合酶-和酶基因与T7启动子序列。该系统显示出比标准系统高2个数量级的效率，该标准系统使用具有劳斯肉瘤病毒启动子pRSVL的CL和荧光素酶基因。此外，使用LLCMK 2细胞的体外实验表明，转染后细胞质基因表达迅速发生（6小时内）。而pRSVL诱导荧光素酶表达需要24-48 h。我们的研究结果表明，细胞质基因表达系统是有用的基因传递到中枢神经系统。
Development of methodologies for gene transfer into the central nervous system (CNS) is important for fundamental research as well as clinical studies for gene therapy. Cationic liposomes (CL) are attractive vectors because of their safety and ease of use. However, to date only low rates of success have been reported. We succeeded in obtaining high transfection efficiencies into the newborn mouse brain in vivo by CL and a cytoplasmic gene expression system based on T7 RNA polymerase and T7 RNA polymerase- and the luciferase-gene with the T7 promoter sequence. This system showed an efficiency rate 2 orders of magnitude higher than the standard system, which used CL and luciferase genes with a Rous sarcoma virus promoter, pRSVL. In addition, in vitro experiments using LLCMK2 cells showed that cytoplasmic gene expression occurred rapidly (within 6 h) after transfection. In contrast, pRSVL required 24-48 h for induction of luciferase expression. Our results suggest that the cytoplasmic gene expression system is useful for gene delivery into the CNS.
脂质体介导的动脉基因转移后基因表达增加，与内膜平滑肌细胞增殖相关。
DOI：
10.1172/jci117017
发表时间：
1994
期刊：
The Journal of clinical investigation
影响因子：
--
作者：
Takeshita,S;Gal,D;Leclerc,G;Pickering,JG;Riessen,R;Weir,L;Isner,JM
通讯作者：
Isner,JM
外源性神经生长因子对大鼠脑中单纯疱疹病毒扩增子载体的神经毒性和神经元基因传递的影响。
影响因子：
4.2
作者：
Pakzaban,P;Geller,AI;Isacson,O
通讯作者：
Isacson,O