HEMOLYTIC-ANEMIA IN NONOBESE DIABETIC MICE
HEMOLYTIC-ANEMIA IN NONOBESE DIABETIC MICE
复制标题
DOI：
10.1002/eji.1830210912
复制
发表时间：
1991-09-01
影响因子：
5.4
通讯作者：
MANDEL, TE
中科院分区：
文献类型：
--
作者：
BAXTER, AG;MANDEL, TE
非肥胖型糖尿病小鼠（NOD小鼠）被广泛用作器官特异性自身免疫的模型，因为其发展由T细胞介导的胰腺β细胞的特异性自身免疫破坏并最终导致胰岛素依赖型糖尿病。 在这里，我们报告，NOD小鼠也发展Coombs '阳性溶血性贫血，B细胞介导的自身免疫性疾病。老年NOD小鼠被发现有脾肿大和黄疸主要是由于升高的未结合血清胆红素。 他们的红细胞压积明显降低，网织红细胞计数也相应增加。 贫血小鼠的红细胞（RBC）对低渗表现出正常的溶解反应。 通过Cr-51标记，来自非贫血小鼠的RBC在非贫血、非糖尿病NOD小鼠中具有正常的半衰期，但在贫血小鼠中显著缩短半衰期。 贫血小鼠的RBC也获得了类似的结果。 贫血小鼠血清可转移溶血作用，导致网织红细胞增多。 直接Coombs试验证实了贫血的抗体介导性质。 仅在> 200天的小鼠中发现贫血，并且在300天时在糖尿病小鼠（4/8）中比非糖尿病小鼠（1/16）更常见。 然而，到第550天，14/17只非糖尿病小鼠受到影响。
The non-obese diabetic mouse (NOD mouse) is widely used as a model of organ-specific autoimmunity because it develops specific autoimmune destruction of pancreatic-beta cells mediated by T cells and culminating in insulin-dependent diabetes mellitus. Here, we report that the NOD mouse also develops Coombs'-positive hemolytic anemia, a B cell-mediated autoimmune disease.Aged NOD mice were found to have splenomegaly and jaundice predominantly due to raised unconjugated serum bilirubin. Their hematocrits were markedly lowered, and there was a reciprocal increase in the reticulocyte count. Red blood cells (RBC) from anemic mice showed a normal lytic response to hypotonicity. RBC from non-anemic mice had normal half lives in non-anemic, non-diabetic NOD mice by Cr-51 labeling but, dramatically shortened half lives in anemic mice. Similar results were obtained with RBC from anemic mice. Hemolysis could be transferred with serum from anemic mice resulting in reticulocytosis. The antibody-mediated nature of the anemia was confirmed with the direct Coombs' test. Anemia was found only in mice aged > 200 days and was more common in diabetic (4/8) than non-diabetic (1/16) mice at 300 days. However, by 550 days, 14/17 non-diabetic mice were affected.