Coil-type implantable glucose biosensor with excess enzyme loading.
Coil-type implantable glucose biosensor with excess enzyme loading.
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DOI：
10.2741/1547
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发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Bazhang Yu;Y. Moussy;F. Moussy
中科院分区：
文献类型：
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作者：
Bazhang Yu;Y. Moussy;F. Moussy
作为设计用于长期皮下植入的葡萄糖传感器的总体长期目标的一部分，我们开发了一种线圈型植入式葡萄糖传感器，该传感器在直径为 0.125 毫米的铂铱丝线圈内装载了过量的葡萄糖氧化酶 (GOD)。过量的 GOD 被固定在用棉花加固的戊二醛/牛血清白蛋白 (BSA) 凝胶中，并位于传感器的线圈室内。过量的 GOD 延长了传感器的使用寿命。基于这种线圈式设计，对采用醋酸纤维素（CA）、聚氯乙烯（PVC）、聚氨酯（PU）、聚（双酚A碳酸酯）（PC）和Nafion外膜的各种线圈式葡萄糖传感器进行了研究和比较。相比之下，基于 Nafion 的生物传感器提供了最佳的长期响应稳定性。然而，Nafion仍然无法满足高酶负载量的线圈型传感器的寿命要求，因为观察到的这些传感器的功能故障确实是由外膜损伤而不是酶活性丧失引起的。其他实验还表明，当传感器未极化时，GOD 浸渍棉中会发生过氧化氢积累，这可能会导致轻微的误报测量。然而，通过使用适当的测量技术可以轻松避免这种伪影。
As part of our overall long-term objective of designing a glucose sensor for long-term subcutaneous implantation, a coil-type implantable glucose sensor loaded with excess glucose oxidase (GOD) inside the coils of a 0.125mm diameter coiled platinum-iridium wire has been developed. The excess GOD was immobilized in a glutaraldehyde/bovine serum albumin (BSA) gel reinforced with cotton and located inside the coils chamber of the sensor. The excess GOD increased the lifetime of the sensor. Based on this coil-type design, various coil-type glucose sensors with cellulose acetate (CA), poly(vinyl chloride)(PVC), polyurethane (PU), poly(bisphenol A carbonate) (PC) and Nafion outer membranes were investigated and compared. Comparatively, Nafion based biosensors provided the best long-term response stability. However, Nafion can still not meet the lifetime requirement of the coil-type sensor with high enzyme loading because the observed function failure of these sensors was indeed caused by outer membrane damage rather than loss of enzyme activity. Additional experiments also revealed that hydrogen peroxide accumulation occurred in the GOD impregnated cotton when the sensors were not polarized which could cause a small false positive measurement. However, this artifact can be easily avoided by using an appropriate measurement technique.