Denervation provokes greater reductions in insulin-stimulated glucose transport in muscle than severe diabetes
Denervation provokes greater reductions in insulin-stimulated glucose transport in muscle than severe diabetes
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DOI：
10.1023/a:1007108025929
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发表时间：
2000-07-01
影响因子：
4.3
通讯作者：
Bonen, A
中科院分区：
文献类型：
--
作者：
Han, XX;Fernando, PK;Bonen, A
我们研究了胰岛素不足(链脲佐菌素(STZ)诱导的糖尿病，85 mg/kg ip)的独立和联合影响。大鼠肌肉(比目鱼肌、红色腓肠肌和白色腓肠肌)基础、胰岛素刺激和收缩刺激的葡萄糖运输时，肌肉活动(失神经)减少(7天)。有四种处理：对照组、失神经肌肉、糖尿病肌肉和失神经+糖尿病肌肉。收缩刺激的葡萄糖转运在所有试验组均有相同程度的降低(接近50%)(p<；0.05)。相比之下，糖尿病动物肌肉中胰岛素刺激的葡萄糖转运减少(18-24%，p<；0.05)比失神经肌肉(40-60%，p<；0.05)和失神经+糖尿病肌肉(40-60%，p<；0.05)小得多。GLUT-4mRNA在失神经+糖尿病肌肉中的下降幅度最大(类似于-75%，p<；0.05)。GLUT-4蛋白在三种实验条件下均有相似程度的降低(P<；0.05)(类似于-30-40%)。总而言之，(1)肌肉不活动(失神经)和STZ诱导的糖尿病在减少收缩刺激的葡萄糖运输方面具有相似的效果，但(2)肌肉不活动(去神经)，而不是严重的糖尿病，在骨骼肌胰岛素刺激的葡萄糖运输中产生的损害是前者的2倍。
We have examined the independent and combined effects of insulin insufficiency (streptozotocin (STZ)-induced diabetes, 85 mg/kg i.p.) and reduced muscle activity (denervation) (7 days) on basal, insulin-stimulated and contraction-stimulated glucose transport in rat muscles (soleus, red and white gastrocnemius). There were four treatments: control, denervated, diabetic, and denervated + diabetic muscles. Contraction-stimulated glucose transport was lowered (similar to 50%) (p < 0.05) to the same extent in all experimental groups. In contrast, there was a much smaller reduction insulin-stimulated glucose transport in muscles from diabetic animals (18-24% reduction, p < 0.05) than in denervated muscles (40-60% reduction, p < 0.05) and in denervated + diabetic muscles (40-60% reduction, p < 0.05). GLUT-4 mRNA reduction was greatest in denervated + diabetic muscles ( similar to -75%, p < 0.05). GLUT-4 protein was decreased (p < 0.05) to a similar extent in all three experimental conditions ( similar to -30-40%). In conclusion, (1) muscle inactivity (denervation) and STZ-induced diabetes had similar effects on reducing contraction-stimulated glucose transport, but (2) muscle inactivity (denervation), rather than severe diabetes, produced a 2-fold greater impairment in skeletal muscle insulin-stimulated glucose transport.