Molecular Determinants for STIM1 Activation During Store- Operated Ca2+ Entry.
Molecular Determinants for STIM1 Activation During Store- Operated Ca2+ Entry.
复制标题
存储操作 Ca2 进入过程中 STIM1 激活的分子决定因素
DOI：
10.2174/1566524017666170220103731
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发表时间：
2017
影响因子：
2.5
通讯作者：
Zhou Y
中科院分区：
文献类型：
--
作者：
Ma G;Zheng S;Ke Y;Zhou L;He L;Huang Y;Wang Y;Zhou Y
STIM/ORAI-mediated store-operated Ca2+ entry (SOCE) mediates a myriad of Ca2+-dependent cellular activities in mammals. Genetic defects in STIM1/ORAI1 lead to devastating severe combined immunodeficiency; whereas gain-of-function mutations in STIM1/ORAI1 are intimately associated with tubular aggregate myopathy. At molecular level, a decrease in the Ca2+ concentrations within the lumen of endoplasmic reticulum (ER) initiates multimerization of the STIM1 luminal domain to switch on the STIM1 cytoplasmic domain to engage and gate ORAI channels, thereby leading to the ultimate Ca2+ influx from the extracellular space into the cytosol. Despite tremendous progress made in dissecting functional STIM1-ORAI1 coupling, the activation mechanism of SOCE remains to be fully characterized. Building upon a robust fluorescence resonance energy transfer assay designed to monitor STIM1 intramolecular autoinhibition, we aimed to systematically dissect the molecular determinants required for the activation and oligomerization of STIM1. Here we showed that truncation of the STIM1 luminal domain predisposes STIM1 to adopt a more active conformation. Replacement of the single transmembrane (TM) domain of STIM1 by a more rigid dimerized TM domain of glycophorin A abolished STIM1 activation. But this adverse effect could be partially reversed by disrupting the TM dimerization interface. Moreover, our study revealed regions that are important for the optimal assembly of heterooligomers composed of full-length STIM1 with its minimal STIM1-ORAI activating region, SOAR. Our study clarifies the roles of major STIM1 functional domains in maintaining a quiescent configuration of STIM1 to prevent preactivation of SOCE.
