Reduced numbers of dopamine neurons in the substantia nigra pars compacta and ventral tegmental area of rats fed an n-3 polyunsaturated fatty acid-deficient diet:: A stereological study
Reduced numbers of dopamine neurons in the substantia nigra pars compacta and ventral tegmental area of rats fed an n-3 polyunsaturated fatty acid-deficient diet:: A stereological study
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DOI：
10.1016/j.neulet.2008.04.073
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发表时间：
2008-06-27
影响因子：
2.5
通讯作者：
Levant, Beth
中科院分区：
文献类型：
--
作者：
Ahmad, S. Omar;Park, Ji-Hyuk;Levant, Beth
膳食n-3多不饱和脂肪酸(PUFA)含量不足与中枢神经系统多巴胺系统的功能改变有关。本研究测定了饲料中n-3多不饱和脂肪酸含量对多巴胺细胞数量和形态的影响。成年(出生后70d)雄性Long-Evans大鼠从受孕开始就在含有足够(对照)或可忽略的n-3PUFAs的饲料中饲养。用体视学方法测定黑质致密部和腹侧被盖区内酪氨酸羟化酶阳性细胞的数目和形态。饲喂n-3多不饱和脂肪酸缺乏饲料的大鼠黑质致密部酪氨酸羟化酶阳性细胞数比对照组减少33.9%，腹侧被盖区减少33.7%(P<；0.05)，但两个脑区酪氨酸羟化酶阳性细胞体的体积无差异。与对照组相比，喂食n-3多不饱和脂肪酸缺乏的大鼠也表现出树突状枯竭和酪氨酸羟基酶阳性细胞的分离，后者有酪氨酸羟基酶阳性细胞的聚集和广泛的树突状树枝。这些发现支持n-3多不饱和脂肪酸在多巴胺神经元存活中的作用，并表明多巴胺细胞数量和功能的改变有助于观察到n-3多不饱和脂肪酸缺乏饮食喂养的大鼠的行为影响。(C)2008爱思唯尔爱尔兰有限公司。保留所有权利。
Inadequate dietary n-3 polyunsaturated fatty acid (PUFA) content is associated with altered function of the CNS dopamine systems. in this study, the effects of dietary n-3 PUFA content were determined on dopamine cell number and morphology. Adult (postnatal day 70), male, Long-Evans rats were raised from conception on diets containing adequate (control) or negligible n-3 PUFAs. The number and morphology of tyrosine hydroxylase-positive cells in the substantia nigra pars compacta and ventral tegmental area were determined stereologically. The number of tyrosine hydroxylase-positive cells in rats fed the n-3 PUFA-deficient diet was 33.9% lower in the substantia nigra pars compacta and 33.7% lower in the ventral tegmental area than in those fed the control diet (P < 0.05); however, the volume of tyrosine hydroxylase-positive cell bodies was not different between diet groups in either brain region. Rats fed the n-3 PUFA-deficient diet also exhibited dendritic depletion and isolation of tyrosine hydroxylase-positive cells compared to rats fed the control diet, which had clustering of tyrosine hydroxylase-positive cells and extensive dendritic arborization. These findings support a role for n-3 PUFAs in the survival of dopamine neurons and suggest that altered dopamine cell number, as well as function, contributes to the behavioral effects observed in rats raised on n-3 PUFA-deficient diets. (c) 2008 Elsevier Ireland Ltd. All rights reserved.