T-CELL AND BASOPHIL ACTIVATION THROUGH THE CYTOPLASMIC TAIL OF T-CELL-RECEPTOR ZETA-FAMILY PROTEINS
T-CELL AND BASOPHIL ACTIVATION THROUGH THE CYTOPLASMIC TAIL OF T-CELL-RECEPTOR ZETA-FAMILY PROTEINS
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DOI：
10.1073/pnas.88.20.8905
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发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
KLAUSNER, RD
中科院分区：
文献类型：
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作者：
LETOURNEUR, F;KLAUSNER, RD
T细胞抗原受体的Zeta链是以二硫键连接的二聚体形式存在的蛋白质家族的原型，是T细胞抗原受体以及与IgE和Ig G结合的Fc受体的亚单位。两个相关基因编码Zeta和Gamma蛋白。在这项研究中，我们检测了由白细胞介素2受体(Tac)的阿尔法链的胞外区和Zeta或Gamma的胞内区组成的嵌合蛋白在T细胞或大鼠嗜碱性白血病细胞中表达时激活细胞的能力。Zeta和Gamma嵌合体在外交联时有效地诱导T细胞产生白细胞介素2和大鼠嗜碱性白血病细胞释放5-羟色胺。构建了Zeta和Gamma的胞质尾部缺失突变体，并用其验证了这些嵌合蛋白激活细胞的特异性。Zeta尾在第108位被截断或从第66位到114位被删除的互补突变体的信号潜力表明，Zeta中存在几个功能结构域。
The zeta-chain of the T-cell antigen receptor is the prototype of a family of proteins that exist as disulfide-linked dimers and are subunits of the T-cell antigen receptor and both IgE and IgG binding Fc receptors. Two related genes encode the zeta and gamma-proteins. In this study we examine the ability of chimeric proteins consisting of the extracellular domain of the alpha-chain of the interleukin 2 receptor (Tac) and the cytoplasmic domain of either zeta or gamma to activate cells when expressed in either T cells or rat basophilic leukemia cells. The zeta and gamma-chimera were effective at eliciting interleukin 2 production in T cells and serotonin release in rat basophilic leukemia cells when externally cross-linked. Cytoplasmic-tail deletion mutants of zeta and gamma were constructed and used to verify the specificity of cell activation by these chimeric proteins. Signaling potencies of complementary mutants having the zeta-tail truncated in position 108 or deleted from positions 66 through 114 suggested the presence of several functional domains in zeta.