Iodination of bacteria: a bactericidal mechanism.
Iodination of bacteria: a bactericidal mechanism.
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DOI：
10.1084/jem.126.6.1063
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发表时间：
1967-12-01
影响因子：
15.3
通讯作者：
Klebanoff, S J
中科院分区：
文献类型：
--
作者：
Klebanoff, S J
髓过氧化物酶、碘化物和H2 O2对大肠杆菌有杀菌作用。在该系统中，髓过氧化物酶可被乳过氧化物酶或豚鼠白细胞颗粒制剂替代，H2 O2可被H2 O2生成系统如葡萄糖和葡萄糖氧化酶替代，碘化物可被甲状腺素或三碘甲状腺原氨酸替代。杀菌效果在pH 5.0时较高，随着pH的升高而下降。髓过氧化物酶，碘化物，和H2 O2的预孵育30分钟前加入的细菌在很大程度上阻止了杀菌作用。因此，在碘化物氧化过程中，生物体必须存在于反应混合物中，以达到最大限度的杀灭，这表明涉及碘化物氧化的不稳定中间体，而不是更稳定的氧化终产物，如碘。碘化的细菌髓过氧化物酶-碘化物-H2 O2系统证明了化学和放射自显影。碘化和杀菌作用同样受到所有测试参数（预孵育、pH值和抑制剂的影响）实验条件变化的影响。豚鼠白细胞对细菌的吞噬作用与碘化物向三氯乙酸可沉淀形式的转化有关。放射自显影法将碘定位于含有摄入细菌的人白细胞的细胞质中。在没有吞噬作用或存在他巴唑的情况下，未观察到碘固定。
Myeloperoxidase, iodide, and H2O2 have a bactericidal effect on Escherichia coli. Myeloperoxidase can be replaced in this system by lactoperoxidase or by a guinea pig leukocyte particulate preparation, H2O2 by an H2O2-generating system such as glucose and glucose oxidase, and iodide by thyroxine or triiodothyronine. The bactericidal effect was high at pH 5.0 and fell as the pH was increased. Preincubation of myeloperoxidase, iodide, and H2O2 for 30 min before the addition of the bacteria largely prevented the bactericidal effect. Thus, the organisms must be present in the reaction mixture during iodide oxidation for maximum killing, which suggests the involvement of labile intermediates of iodide oxidation rather than the more stable end products of oxidation such as iodine. Iodination of the bacteria by the myeloperoxidase-iodide-H2O2 system was demonstrated chemically and radioautographically. Iodination and the bactericidal effect were similarly affected by changes in experimental conditions in all the parameters tested (effect of preincubation, pH, and inhibitors). Phagocytosis of bacteria by guinea pig leukocytes was associated with the conversion of iodide to a trichloroacetic acid-precipitable form. Iodide was localized radioautographically in the cytoplasm of human leukocytes which contained ingested bacteria. Iodide fixation was not observed in the absence of phagocytosis or in the presence of Tapazole.